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| PCR-SBT法检测北京地区人群HLA-DRB1遗传多态性 | |
| 引用本文： | 邓亚军,杨光,吴东颖,胡松年,李生斌,朱波峰,朱军,刘耀. PCR-SBT法检测北京地区人群HLA-DRB1遗传多态性[J]. 法医学杂志, 2005, 21(4): 280-282,285 |
| 作者姓名： | 邓亚军 杨光 吴东颖 胡松年 李生斌 朱波峰 朱军 刘耀 |
| 作者单位： | 1. 西安交通大学卫生部法医学重点实验室,陕西,西安,710061;中国科学院北京基因组研究所,北京,101300;北京华大方瑞司法物证鉴中心 2. 西安交通大学卫生部法医学重点实验室,陕西,西安,710061;中国科学院北京基因组研究所,北京,101300 3. 中国科学院北京基因组研究所,北京,101300;北京华大方瑞司法物证鉴中心,北京 101300 4. 北京华大方瑞司法物证鉴中心,北京 101300 5. 西安交通大学卫生部法医学重点实验室,陕西,西安,710061 6. 西安交通大学卫生部法医学重点实验室,陕西,西安,710061;公安部物证鉴定中心,北京,100038 |
| 摘 要： | 目的调查北京地区人群HLA-DRB1基因座多态性,并探讨HLA的聚合酶链反应-直接测序分型(PCR-SBT)在法医物证学中的应用价值。方法应用PCR-SBT分型方法对北京地区人群中494名健康无关个体进行HLA-DRB1基因座高分辨分型。结果检出233种HLA-DRB1基因型、102种DRB1等位基因型,基因型的分布符合Hardy-Weinberg平衡定律,该位点的观察杂合度(Ho)为0.9210,期望杂合度(He)为0.9342,多态信息量(PIC)为0.9333,匹配概率(Pm)为0.0104,个体识别率(DP)为0.9896,非父排除率(PPE)为0.7776。结论使用PCR-SBT对HLA进行精确分型在法医学领域具有重要的应用价值。 |
| 关 键 词： | 基因频率 法医学 |
| 文章编号： | 1004-5619(2005)04-0280-03 |
| 修稿时间： | 2005-03-08 |
Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region |
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| Deng Ya-jun,,Yang Guang,,Wu Dong-ying,et al.. Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region[J]. Journal of Forensic Medicine, 2005, 21(4): 280-282,285 | |
| Authors: | Deng Ya-jun Yang Guang Wu Dong-ying et al. |
| Affiliation: | Deng Ya-jun1,2,Yang Guang1,2,Wu Dong-ying2,et al. |
| Abstract: | Objective To investigate the polymorphism of HLA-DRB1 gene in a population of Beijing region and research on the application feasibility of HLA-DRB1 by PCR-SBT method. Methods Polymerase chain reaction sequence-based typing (PCR-SBT) method was used to determine HLA-DRB1 genotypes of 494 unrelated healthy people of Beijing region. Results A total of 233 different HLA-DRB1 genetypes and 102 alleles were detected. The observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), probability of matching (Pm); discrimination power (DP) and probability of paternity exclusion (PPE) of HLA-DRB1 in the samples were calculated and their values were 0.921 0,0.934 2, 0.933 3, 0.010 4, 0.989 6,and 0.777 6 respectively. Conclusion HLA-DRB1 typing by PCR-SBT is very useful in forensic science. |
| Keywords: | HLA-DRB1 PCR-SBT |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |