| 微量体表脱落上皮细胞的DNA检验 |
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| 引用本文： | 张幼芳,李佑英. 微量体表脱落上皮细胞的DNA检验[J]. 法律与医学杂志, 2003, 10(2): 110-112 |
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| 作者姓名： | 张幼芳 李佑英 |
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| 作者单位： | 浙江公安高等专科学校 杭州310053(张幼芳)，浙江省杭州市公安局刑侦支队 310004(李佑英) |
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| 基金项目： | 浙江省公安厅 2 0 0 1年度立项课题编号 2 0 0 1- 0 3 |
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| 摘 要： | 目的 建立微量体表脱落上皮细胞的DNA检验方法。 方法 采用Chelex -10 0法提取DNA ,以Microcon -10 0纯化柱纯化浓缩DNA ,用ProfilerPlus试剂盒PCR扩增后用 3 10基因分析仪检测。 结果 10种常见粘附有体表脱落上皮细胞的样本 10 0个 ,其中 7种 70个样本成功检测到 10个STR位点的分型 ,检出率为 10 0 % ,其余 3种样本检出率分别为 5 0 %、2 0 %、10 % ,将该方法应用于 2例实际检案 ,取得满意效果。 结论 所建立方法稳定可靠 ,易于操作 ,适用于多种检材 ,为微量体表脱落上皮细胞的DNA检验提供了确实可行的检验方法
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| 关 键 词： | 表皮细胞 STR DNA提取 |
| 文章编号： | 1007-9297(2003)02-0110-03 |
| 修稿时间： | 2002-11-28 |
Examination of DNA from dropped macro-epidermis cells. |
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| ZHANG You fang,LI You ying. Zhejiang public security college,Zhejiang province,PRC. Examination of DNA from dropped macro-epidermis cells.[J]. The Journal of Law & Medicine, 2003, 10(2): 110-112 |
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| Authors: | ZHANG You fang LI You ying. Zhejiang public security college Zhejiang province PRC |
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| Affiliation: | ZHANG You fang,LI You ying. Zhejiang public security college,Zhejiang province,PRC,310053 |
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| Abstract: | Objective To establish a method for testing of DNA from dropped macro-epidermis cells. Methods 100 samples in ten types of added dropped epidermis cells were collected in this study. The DNA of dropped epidermis cells was extracted by Chelex-100, and purified and concentrated by using Microcon-100. These sample was examined using Profiler Plus kit with ABI310 genetic analyzer. Two criminal cases was applied as blind-test. Results The 10 STR loci in 70 samples of seven types were detected successfully. In addition, the rate of STR detection in other types samples were 50%, 20% and 10% respectively. The satisfaction results was also obtained in two criminal cases. Conclution The results showed that it was a reliable method to extract and test DNA from dropped macro-epidermis cells. |
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| Keywords: | Epidermis Cells STR DNA Extraction |
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