| 伪狂犬病病毒gG-gD基因片段的扩增及克隆和序列测定 |
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| 引用本文： | 罗满林,黄毓茂,刘镇明,吴红专,袁少华. 伪狂犬病病毒gG-gD基因片段的扩增及克隆和序列测定[J]. 中国兽医科学, 2001, 31(8): 3-5 |
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| 作者姓名： | 罗满林 黄毓茂 刘镇明 吴红专 袁少华 |
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| 作者单位： | 华南农业大学动物医学系,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金资助项目(39770033);广东省科技攻关项目(2000-2001)的部分内容 |
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| 摘 要： | 以伪狂犬病病毒(PRV)HB-9304株的基因组为模板,利用聚合酶链反应(PCR)对其gG-gD基因片段进行扩增,获得了预定大小的片段,将这一片段克隆到质粒载体pPK中.对重组质粒pPKGD进行限制性内切酶分析、PCR鉴定和克隆片段的序列测定,证实了克隆片段的可靠性.
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| 关 键 词： | 伪狂犬病病毒 聚合酶链反应 gG-gD基因片段 分子克隆 序列测定 |
| 文章编号： | 1000-6419(2001)08-0003-03 |
| 修稿时间： | 2001-03-09 |
Amplification, Cloning and Sequencing of a Fragments of G and D Gene of Pseudorabies Virus |
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| LUO Man lin,HUANG Yu mao,LIU Zhen ming,WU Hong zhuan,YUAN Shao hua. Amplification, Cloning and Sequencing of a Fragments of G and D Gene of Pseudorabies Virus[J]. Chinese Veterinary Science, 2001, 31(8): 3-5 |
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| Authors: | LUO Man lin HUANG Yu mao LIU Zhen ming WU Hong zhuan YUAN Shao hua |
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| Abstract: | Using the genome DNA of the strain HB 9304 of Pseudorabies virus (PRV) as template, the fragments flanking gG and gD were amplified by polymerase chain reaction(PCR) .The expected size of fragments were obtained ,and then cloned into the plasmid pPK. The recombinant plasimd of pPKGD was identified by restriction enzyme analysis,PCR and sequencing, which proved completely its validity. |
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| Keywords: | pseudorabies viurs PCR a fragments of G and D gene molecular clone sequencing |
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