人脾淋巴细胞核DNA含量及形态学参数的变化规律

目的 探讨人体死亡后脾淋巴细胞核DNA含量及形态学参数的变化规律。方法 选取具有明确死亡时间的人体尸体材料,离体脾脏置于室内(19～24℃),在40h内每3～7h取材1次,做成细胞悬液,经RNA酶水解等处理,PI染色,流式细胞仪测定细胞核中含不完整DNA的细胞数,获得M1值。结果 随着死亡时间的延长,M1值逐渐增大,与死亡时间具有明显的相关性,其相关系数为0.9826。在21h,M1值达21.146％,其后M1值的增大出现加速的趋势,在40h达56.965％,并仍有增大的趋势。结论 人体脾脏淋巴细胞核DNA的降解情况(M1值)与死亡时间具有明显的相关性,延长取材时间,积累标本,建立数据库,将为死亡时间的推断提供较为精确的方法。

The degradation of nuclear DNA of lymphocytes in human spleen as the PMI lasted

Objective To search the rule of DNA degradation in nuclei of human cadaver. Method Five cadavers with known time of death were selected. The spleens were isolated, set in the room, covered with moisten fabric and were sampled every 4h to 7h within 40h period. Cell suspensions were made, digested by Rnase, stained with PI, analyzed by FACS-sort Flow Cytometry (FSM). Cells contained fragment DNA were counted. And the ration (Mj) were then obtained. Results Along with the prolongation of the PMI, the value of MI was increased. There was a definite relationship between the M1 and the post-morten interval (PMI). At the time of 21th hour, the value of Mt was 21.146% , and the augment of M1 was accelerated. At the time of 40th hour, the value of MI was 56.9565% and the augment was still continued. This is the first report of this kind study in our country. Conclusion After death, the degradation of the nuclear DNA of lymphocyte in human spleen (Mi) has a definite relationship with PMI. Accumulation of samples and data will provide a precise and simple way for determing the PMI.