Derivative spectrophotometric studies on cytotoxic effects of carbon monoxide |
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| Authors: | Y Fukui A Akane S Takahashi K Matsubara C Maseda |
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| Affiliation: | 1. Department of Forensic Sciences, School for Bioengineering and Biosciences Sciences, Lovely Professional University, Phagwara, India;2. Department of Chemistry, School for Chemical Engineering and Physical Sciences, Lovely Professional University, Phagwara, India;1. Beckman Institute for Advanced Science and Technology, 405 N. Mathews Ave., Urbana, IL 61801, USA;2. Center for Nutrition, Learning and Memory, University of Illinois at Urbana-Champaign, USA;3. Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, PO Box 226, RG2 6AP, Reading, UK;1. Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education; School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China;2. Centre for Optical and Electromagnetic Research, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, PR China;3. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, 518060, PR China;4. Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem 636 011, Tamil Nadu, India;5. Centre for Sustainable Nanomaterials, Sina Institute for Scientific and Industrial Research (ISI-SIR), Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia;1. Faculty of Medicine, University of São Paulo, São Paulo, Brazil;2. Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil;3. Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, Brazil;4. Department of Pharmacy, Anhanguera University of São Paulo, São Paulo, Brazil;5. Department of Biotechnology, Anhanguera University of São Paulo, São Paulo, Brazil;1. Toxicology Unit, Department of Clinical Biochemistry, King''s College Hospital NHS Foundation Trust, London SE5 9RS, UK;2. Toxicology Unit, Dept of Pathology, Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK |
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| Abstract: | Characteristics of cytochrome oxidase prepared from hearts of Sprague-Dawley male rats were studied with the use of the fourth derivative spectrophotometry in respect to the cytotoxic effects of carbon monoxide (CO). CO-exposed rats tended to show lower specific activities of cytochrome oxidase than control and recovered rats. Moreover, there was a significant difference in the fourth derivative spectral features of the enzyme: CO-exposed groups indicated peaks at 412 nm in the spectra while controls at 408 nm. This spectral difference seemed to reflect specific effect of CO on cytochrome oxidase, though such trace remained for not more than a day after death. |
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