| 猫源弓形虫虫株的分离与鉴定 |
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| 引用本文： | 张翰,周东辉,陈强,唐剑栋,宋慧群,朱兴全. 猫源弓形虫虫株的分离与鉴定[J]. 中国兽医科学, 2007, 37(2): 160-162 |
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| 作者姓名： | 张翰 周东辉 陈强 唐剑栋 宋慧群 朱兴全 |
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| 作者单位： | 华南农业大学,兽医学院兽医寄生虫研究室,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金;广东省自然科学基金 |
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| 摘 要： | 将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小白鼠,经连续传代分离弓形虫虫株,用特异PCR方法对所分离的虫株进行了鉴定。分别从24只猫的样品中分离出8株弓形虫虫株,用弓形虫种特异的PCR方法对8个虫株进行鉴定,均得到弓形虫的特异条带;测序证明所扩增出的DNA片段确为弓形虫的核糖体DNA第一内转录间隔区(ITS-1)部分序列。研究结果表明,将动物组织用盐酸-胃蛋白酶溶液消化处理后接种小白鼠是一种分离弓形虫虫株较为理想的方法,特异PCR方法能准确、快速地鉴定弓形虫虫株。
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| 关 键 词： | 弓形虫 猫 虫株分离 特异PCR 鉴定 |
| 文章编号： | 1673-4696(2007)02-0160-03 |
| 修稿时间： | 2006-12-18 |
Isolation and identification of Toxoplasma gondii strains from cats |
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| ZHANG Han,ZHOU Dong-hui,CHEN Qiang,TANG Jian-dong,SONG Hui-qun,ZHU Xing-quan. Isolation and identification of Toxoplasma gondii strains from cats[J]. Chinese Veterinary Science, 2007, 37(2): 160-162 |
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| Authors: | ZHANG Han ZHOU Dong-hui CHEN Qiang TANG Jian-dong SONG Hui-qun ZHU Xing-quan |
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| Abstract: | The objective of the present study was to isolate Toxoplasma gondii strains from cats and then to identify these strains by specific PCR assay.The T.gondii strains were isolated by inoculation in Kunming mice with cat tissues(the heart,brain and tongue) which were digested by acid pepsin solution,followed by specific amplification of partial ITS-1 rDNA using species-specific primers.In total,eight T.gondii strains were isolated from 24 cats,and the validity of the strains was confirmed by sequencing of the specific PCR products.These results demonstrated that the method of inoculation in the mice with the tissues that was digested by acid pepsin solution is an effective way to isolate T.gondii strain from animals,and the specific PCR assay is an accurate method for the rapid identification of T.gondii. |
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| Keywords: | Toxoplasma gondii cat isolation specific PCR assay identification |
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