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| 禽脑脊髓炎病毒VR株衣壳蛋白基因的克隆及序列分析 | |
| 引用本文： | 张二芹,赵振华,孙明,赵心力,陈西钊,钱爱东,王金玲. 禽脑脊髓炎病毒VR株衣壳蛋白基因的克隆及序列分析[J]. 中国兽医科学, 2004, 34(3): 51-55 |
| 作者姓名： | 张二芹 赵振华 孙明 赵心力 陈西钊 钱爱东 王金玲 |
| 作者单位： | 1. 内蒙古农业大学,内蒙古,呼和浩特,010018 2. 农业部兽医诊断中心,北京,100094 3. 内蒙古自治区兽医站,内蒙古,呼和浩特,010018 4. 吉林农业大学,吉林,长春,130118 |
| 基金项目： | 内蒙古自治区教育厅重点攻关项目 |
| 摘 要： | 用冻存的禽脑脊髓炎病毒VanRoekel(AEVVR)株通过SPF鸡胚传代复壮 ,获得了试验用病毒株样本。根据已知禽脑脊髓炎病毒 114 3(AEV 114 3)株的衣壳蛋白VP0、VP3、VP1基因序列 ,设计了 3对引物 ,分别扩增AEVVR株的VP0、VP3、VP1基因 ,将RT PCR产物进行分子克隆和序列分析。结果表明 ,AEVVR株与AEV 114 3株VP0、VP3、VP1基因的核苷酸同源性分别为 95 .1%、93.6 %和 93.9% ;氨基酸的同源性分别为 97.5 %、97.5 %和 99.6 %。 |
| 关 键 词： | 禽脑脊髓炎病毒 VP基因 分子克隆 序列分析 同源性 |
| 文章编号： | 1000-6419(2004)03-0051-05 |
| 修稿时间： | 2003-10-29 |
Cloning and sequence analysis of VP genes of avian encephalomyelitis VR strain |
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| ZHANG Er-qin. Cloning and sequence analysis of VP genes of avian encephalomyelitis VR strain[J]. Chinese Veterinary Science, 2004, 34(3): 51-55 | |
| Authors: | ZHANG Er-qin |
| Affiliation: | ZHANG Er-qin~ |
| Abstract: | The experimental avian encephalomyelitis virus (AEV) strain was obtained by means of AEV being passaged in the SPF avian embryos. According to the published sequences of VP0, VP3 and VP1 genes of AEV strain 1143, 3 pairs of primers were designed. The cloning and sequence analysis of the VP0, VP3 and VP1 genes of AEV VR strain was carried out after the VP0, VP3 and VP1 genes of AEV VR strain were amplified respectively with the 3 pairs of primers by RT-PCR. The results showed that the VP0, VP3 and VP1 genes of AEV VR strain shared respectively 95.1%,93.6% and 93.9% homology with those from AEV strain 1143, and that the deduced amino acids sequences from the VP0,VP3 and VP1 genes of AEV VR strain shared respectively 97.5%,97.5% and 99.6% homology with those from AEV strain 1143. |
| Keywords: | avian encephalomyelitis virus VP gene molecular cloning sequence analysis homology |
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