| 丝状霉形体丝状亚种SC型脂蛋白LppQ基因的序列分析 |
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| 引用本文： | 高玉龙,李媛,王砚范,辛九庆. 丝状霉形体丝状亚种SC型脂蛋白LppQ基因的序列分析[J]. 中国兽医科学, 2003, 33(10): 27-30 |
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| 作者姓名： | 高玉龙 李媛 王砚范 辛九庆 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家科技攻关重大专项资助项目 (2 0 0 2BA5 18A0 4 ) |
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| 摘 要： | 根据基因库发表的丝状霉形体丝状亚种SC型 (MmmSC)脂蛋白LppQ基因序列设计引物 ,采用PCR对国内MmmSC分离株HVRI X株的脂蛋白LppQ基因进行扩增 ,将扩增产物与pMD18 T载体连接并测序。核苷酸序列比较结果显示 ,HVRI X株的LppQ基因序列与基因库发表的核苷酸序列同源性为 99.8% ;由其推导的氨基酸序列同源性为 99.3%。
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| 关 键 词： | 牛传染性胸膜肺炎 丝状霉形体丝状亚种SC型 脂蛋白 序列分析 |
| 文章编号： | 1000-6419(2003)10-0027-05 |
| 修稿时间： | 2003-09-26 |
Sequence analysis of LppQ gene of Mycoplasma mycoides subsp. mycoides SC lipoprotein |
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| GAO Yu long,LI Yuan,WANG Yan fan,XIN Jiu qing. Sequence analysis of LppQ gene of Mycoplasma mycoides subsp. mycoides SC lipoprotein[J]. Chinese Veterinary Science, 2003, 33(10): 27-30 |
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| Authors: | GAO Yu long LI Yuan WANG Yan fan XIN Jiu qing |
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| Abstract: | The LppQ gene is specific to M. mycoides subsp. mycoides SC (MmmSC) and was found in field strains isolated in Europe, Africa, as well as in vaccinal strains. The LppQ N terminal domain possessed strong immunogenicity and induced a strong, specific, early, and persistent immune response in naturally and experimentally infected cattle. The LppQ gene was amplified from MmmSC HVRI X strain by PCR using the primer pairs designed according to the reported MmmSC LppQ sequence. It was cloned and sequenced subsequently. The result revealed that there was 99.8% identical in nucleotide sequence, 99.3% identical in induced amino acid sequence , respectively. Molecular cloning of MmmSC LppQ gene makes it possible to express the LppQ gene in vitro. |
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| Keywords: | contagious bovine pleuropneumonia (CBPP) MmmSC lipoprotein sequence analysis |
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