| 人精浆中A型血型物质的分离纯化及理化性质研究 |
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| 引用本文： | 王保捷,李春梅,李庆生,肖仰哲,张核子,卢岩.人精浆中A型血型物质的分离纯化及理化性质研究[J].中国法医学杂志,1998(1). |
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| 作者姓名： | 王保捷 李春梅 李庆生 肖仰哲 张核子 卢岩 |
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| 作者单位： | 中国医科大学法医学系!沈阳，110001，中国医科大学法医学系!沈阳，110001，中国医科大学法医学系!沈阳，110001，中国医科大学法医学系!沈阳，110001，中国医科大学法医学系!沈阳，110001，中国医科大学法医学系!沈阳，110001 |
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| 基金项目： | 国家教委归国人员基金，辽宁省博士启动基金 |
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| 摘 要： | 应用凝胶过滤、阴离子高速液相色谱及免疫亲合层析方法成功地从A型分泌型人精浆中分离、纯化了具有A型活性的精浆血型物质（SPBGS－A）。理化分析结果表明：SPBGS－A为表现单一A型活性、分子量不均一的糖蛋白。其分子量约为100KD；等电点在pH2．7～3．5之间；糖含量占72．5％；蛋白质含量占24．2％。共检出了16种氨基酸成分，其中苏氨酸、丝氨酸及脯氨酸的含量约占50％。经与其它水溶性血型物质在化学组成上进行比较，提示其可能具有一定的器官特异性。
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| 关 键 词： | 精浆 血型物质 纯化 器官特异性 |
Studies of the isolation, purification and physico-chemical characterization of human saminal plasma blood groop substance A(spbgs-A) |
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| Wang Baojie,et al..Studies of the isolation, purification and physico-chemical characterization of human saminal plasma blood groop substance A(spbgs-A)[J].Chinese Journal of Forensic Medicine,1998(1). |
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| Authors: | Wang Baojie |
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| Abstract: | Active SPBGS-A was successfully isolated and purified from seminal plasma of A secretory type individuals by procedures involving gel filtration,anion exchange colqmn chromatography with FPLC system and immunoaffinity chromatography. Physico-chemical analysis suggested that SPBGS-A showed only A cativity,and was a kind of glycoproteins of high heterogeneity. Molecular weight of SPBGS-A was approximately 50 to 70KD and isoelectric point was 2. 7 to 3. 5. It contained nealy 72. 5 % of carbohydrate and 24. 2 % of protein in which Thr, Ser and Pro amouted to 50% of total 16 kinds of amino acid. The result of comparison between it and other water-soluble blood group substances indicated that SPBGS-A might possess organ specificity. |
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| Keywords: | Seminal plasma Blood group substance Purification organ specificity |
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