冠心病猝死者心肌中SOCS-1和Bax的表达

目的采用免疫组化方法,观察细胞因子信号转导抑制因子-1(SOCS-1)和Bax在冠心病猝死(SCD)者心肌中的表达情况,探讨其对SCD诊断的意义。方法 25例诊断为SCD者心脏样本为实验组,25例非心血管疾病猝死者心脏样本为对照组。应用免疫组织化学方法检测SOCS-1和Bax在心肌中的表达,应用SPSS 17.0软件进行统计处理,组间比较采用秩和检验。结果 SCD猝死者心肌SOCS-1与Bax的表达明显高于对照组,Uc值SOCS-1为5.830 6,Bax为5.573,两种指标组间比较,差异均有显著性意义(P<0.01);SCD组中SOCS-1表达阳性以上且Bax表达弱阳性以上有22例,而正常对照组仅有1例。结论 SOCS-1与Bax均可能参与了细胞凋亡的过程,检测两种指标在心肌中阳性表达,可以为SCD的诊断提供客观依据,且联合检测可提高SCD诊断的特异性。

Expressions of suppressor of cytokine signaling-1 and Bcl-2 associated X protein in myocardium of patients underwent sudden coronary death

Objective By using the immunohistochemical method,to observe the expressions of suppressor of cytokine signaling-1(SOCS-1)and Bcl-2 associated X protein(Bax) in the myocardium of patients of sudden coronary death and to explore the significance of SCD diagnosis.Methods The heart samples of twenty-five cases of sudden coronary death were chosen as experiment group while the heart samples of twenty-five cases of non sudden coronary death were chosen as control group.Immunohistochemical staining was employed to examine the expressions of SOCS-1 and Bax in the myocardium,the SPSS 17.0 software for statistical process was applied,the rank-sum test was used in the comparisons between the two groups.Results The expressions of SOCS-1 and Bax in experiment group was significantly higher than those in control group,the Uc value of SOCS-1 was 5.830 6,Bax was 5.573,two indicators between two groups were compared,the differences were statistically significant(P <0.01).The number of cases with positive SOCS-1 expression and weak positive Bax expression in the myocardium in experiment group was 22,while those in control group was only 1.Conclusion SOCS-1 and Bax could participate in the process of apoptosis,the detection of the positive expressions of two indicators in the myocardium may provide an objective basis for the diagnosis of SCD,and the diagnostic specificity of SCD will be improved with their combined application.