| UPLC-MS/MS测定全血中的氯丙嗪 |
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| 引用本文： | 董颖,杜鸿雁,邵永军. UPLC-MS/MS测定全血中的氯丙嗪[J]. 中国法医学杂志, 2013, 28(1): 44-45,48 |
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| 作者姓名： | 董颖 杜鸿雁 邵永军 |
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| 作者单位： | 1. 公安部物证鉴定中心,北京,100038
2. 无锡市公安局刑事科学技术研究所,江苏无锡,214002 |
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| 摘 要： | 目的建立全血中氯丙嗪的UPLC-MS/MS分析方法。方法采用乙腈沉淀蛋白,以Waters ACQUITY UPLCBEH C18柱(2.1mm×50mm,1.7μm)分离,乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,正离子方式检测,多反应离子监测模式(MRM)。结果血液中氯丙嗪在1～100ng/mL范围内线形关系良好,检出限为0.01ng/mL,回收率为81.81%～89.36%,日内、日间精密度分别为9.3%、12.1%。结论本方法准确、快速,可用于全血中氯丙嗪的定性定量分析。
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| 关 键 词： | 法医毒物分析 UPLC-MS/MS 全血 氯丙嗪 |
Determination of chlorpromazine in whole blood by UPLC-MS/MS |
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| Dong Ying,Du Hongyan,Shao Yongjun. Determination of chlorpromazine in whole blood by UPLC-MS/MS[J]. Chinese Journal of Forensic Medicine, 2013, 28(1): 44-45,48 |
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| Authors: | Dong Ying Du Hongyan Shao Yongjun |
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| Affiliation: | 1.Institute of Forensic Science Ministry of Public Security,Beijing 100038,China;2.Public Security Bureau,Jiangsu Wuxi,214002,China) |
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| Abstract: | Objective To establish UPLC-MS/MS method for the determinate on of chlorpromazine in whole blood.Methods Samples were extracted by acetonitrile.Waters ACQUITY UPLC BEH C18 column(2.1mm×50mm,1.7μm) and the mobile phase with acetonitrile:formic acid of 0.1% were used.The samples were detected by electrospray tandem mass spectrometry with ESI+ and multiple reaction monitoring(MRM) modes.Results There was a good linear relationship with in the range of 1～100ng/mL.The limit of detection was 0.01ng/mL.The recoveries were between 81.81% and 89.36%.The within-day and day-to-day RSD ranged from 9.3% to 12.1%.Conclusions The method is simple,rapid,accurate.It can be used for the determination of Chlorpromazine in whole blood. |
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| Keywords: | forensic toxicology analysis UPLC-MS/MS whole blood chlorpromazine |
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