血小板同种抗原基因中9个单核苷酸多态性在广西地区壮族和汉族人群中的差异分析

目的检测血小板同种抗原基因中9个单核苷酸多态性在广西地区壮族和汉族人群中的差异。方法利用基于单碱基延伸的单核苷酸多态性（SNP）分型芯片,对广西壮族地区99例壮族个体和107例汉族个体的染色体基因组上10个SNP位点进行了分型,其中9个位于6个血小板同种抗原基因中,1个位于基因间区。此外,结合Hapmap计划第二期公布的四个人群的SNP分型数据,分析这六个人群的遗传结构。结果广西原住汉族人在等位基因频率上未检测到与当地壮族有显著性的差异位点,但在基因型频率上,rs630014和rs9441951两位点是显著差异的。广西壮族人与广西汉族、北京汉族人及日本东京人的遗传结构相近,但与祖先来自欧洲西部和北部的犹他州居民以及尼日利亚伊巴丹的约鲁巴人有显著差异的遗传成分存在。结论壮汉两族由于历史上的多次基因交流可能导致其遗传信息在很大程度上是相近的。

The Difference Analyses of 10 SNPs in HPA Gene Between Zhuang and Han in Guang Xi Area

Objective To explore the difference of 10 SNPs in HPA gene between Zhuang and Han in Guang Xi area. Methods Ten SNPs were genotyped in 99 Zhuang individuals and 107 Han individuals. Nine of the ten SNPs located in the 6 human platelet alloantigen （HPA） coding genes, and the other one located in the inter-gene region. Analyses were conducted on the differences of ＂allele frequencies and genotype frequencies between Han and Zhuang in Guang Xi area. Moreover, the genetic structure was analyzed for the two populations along with the four populations from Hapmap II data. Results There are no significant allele frequency differences of the 10 SNP loci between Zhuang and Han population in Guang Xi area, while the significant difference of the genotype frequency are shown on rs630014 and rs9441951. The genetic structure of Guang Xi Zhuang, Guang Xi Han, Beijing Han and JPT are similar, while significant genetic structure difference is implied from CEU and YRI. Conclusion The frequent gene communication in history between Zhuang and Han in Guang Xi area could cause the genetic similarity in the two populations.