| 1483例亲子鉴定STR基因座突变的分析 |
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| 引用本文： | 帅莉,汪军,景强,许彬,苏琴,李芸娟,李辉. 1483例亲子鉴定STR基因座突变的分析[J]. 法医学杂志, 2014, 0(1): 44-46 |
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| 作者姓名： | 帅莉 汪军 景强 许彬 苏琴 李芸娟 李辉 |
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| 作者单位： | 昆明市延安医院;昆明法医院司法鉴定中心;昆明医科大学法医学院; |
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| 摘 要： | 目的观察和分析PowerPlex18D试剂盒17个STR基因座在云南人群亲子鉴定中的突变现象。方法应用Chelex-100法提取DNA，采用PowerPlex18D试剂盒检测1483例亲子鉴定结论为“肯定”的案例进行基因分型。结果1483例中双亲三联体亲子鉴定1047例．单亲二联体亲子鉴定436例．总共观察到2530次减数分裂。1483例中共观察到24例有1个STR基因座发生突变。在17个STR基因座中观察到11个基因座存在突变现象。结论STR基因座突变是较为常见的现象，应不断积累STR基因座突变数据，选择其他多态性好、突变率低的遗传标记．以保证鉴定结果的准确可靠。
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| 关 键 词： | 法医遗传学 突变 亲子关系 云南 |
1483 Cases of Paternity Test with STR Loci Mutation |
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| SHUAI Li,WANG Jun,JING Qiang,XU Bin,SU Qin,L,Yun-juan,LI Hui. 1483 Cases of Paternity Test with STR Loci Mutation[J]. Journal of Forensic Medicine, 2014, 0(1): 44-46 |
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| Authors: | SHUAI Li WANG Jun JING Qiang XU Bin SU Qin L Yun-juan LI Hui |
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| Affiliation: | 1. Yah＇an Hospital of Kunming, Kunming 650051, China; 2. Center of Forensic Science, Ktmming Foren- sic Hospital, Kunming 650031, China; 3. School of Forensic Medicine, Kunming Medical University, Kun- ming 650.500, China) |
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| Abstract: | Objective To observe and analyze the mutation phenomenon of 17 STR loci of PowerPlex 18D Kit in paternity test of Yunnan population. Methods The DNA was extracted by Chelex-100 method. The PowerPlex 18D Kit was used to test 1 483 cases and their conclusions of paternity tests were verified. Results In the 1 483 cases, 1 047 were parental triplet and 436 were uniparental diad. A total of 2 530 times of meiosis was observed. One STR locus mutation was observed in 24 cases. And 11 mutation loci were found in the 17 STR loci. Conclusion STR loci mutation is a common phenomenon. We should collect the data of STR loci mutation, choose other good polymorphism, low mutation rate of genetic markers, to ensure that the results are accurate and reliable. |
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| Keywords: | forensic genetics mutation parent-child relations Yunnan |
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