改良差异裂解法结合硅珠法提取混合斑精子DNA

目的采用改良差异裂解法结合硅珠法提取混合斑中精子DNA，并评价其应用价值。方法收集52例经常规差异裂解法检验含有女性分型的混合斑检材，采用改良差异裂解法结合硅珠法提取精子细胞DNA，IdentifilerTM试剂盒进行PCR扩增检验。并将常规差异裂解法结果作为对照。结果52例混合斑检材中，采用改良差异裂解法结合硅珠法检出单一男性精子成分有38例，男性分型检出率达到98．08％。结论改良差异裂解法结合硅珠法适合提取混合斑中精子DNA。

Extraction of Sperm DNA from Mixed Stain by the Modified Differential Lysis Method Combined with Silicon Bead Method

Objective To extract sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method and to evaluate its application value. Methods Fifty-two mixed stains containing female STR genotypes detected by differential lysis method were collected. The sperm DNA was extracted by the modified method combined with silicon bead method, then genotyped with the IdentifilerTM Kit, and compared with the results of genotyping by the conventional differential lysis method as control. Results Of the 52 samples, 38 samples with sole male STR genotypes in all loci were detected. The detection rate of 1male STR genotypes was 98.08% through the modified method combined with silicon bead method. Conclusion The modified differential lysis method combined with silicon bead method can be used in extraction of sperm DNA from mixed stain.