| 大鼠脑挫伤后基质金属蛋白酶3的表达 |
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| 引用本文： | 郭晓冲,李如波,梁红霞,王福远,吴旭. 大鼠脑挫伤后基质金属蛋白酶3的表达[J]. 法医学杂志, 2009, 25(1): 1-5. DOI: 10.3969/j.issn.1004-5619.2009.01.001 |
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| 作者姓名： | 郭晓冲 李如波 梁红霞 王福远 吴旭 |
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| 作者单位： | 1. 中国医科大学,法医学院法医病理学教研室,辽宁,沈阳,110001;中国医科大学,实验动物部,辽宁省实验动物转基因重点实验室,辽宁,沈阳,110001
2. 中国医科大学,法医学院法医病理学教研室,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省高等学校科研项目 |
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| 摘 要： | 目的 检测脑挫伤修复过程中基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达,探讨其表达规律与损伤时间的相关性.方法 将大鼠分成对照组、假手术组和脑挫伤组.应用免疫组织化学技术和蛋白质印迹(Western blot)方法检测脑挫伤后不同时间脑组织中MMP-3的表达变化. 结果免疫组化结果:对照组及假手术组脑组织未见MMP-3阳性染色;伤后6h组脑组织出现MMP-3阳性染色,此后24h内染色逐渐增强,伤后5 d阳性细胞数及染色强度均达高峰,随之下降,伤后14d仍有少量MMP-3表达.Western blot结果:对照组及假手术组脑组织未见MMP-3阳性染色;挫伤后6h组出现MMP-3表达,随后表达量逐渐上升,5d达到高峰,以后逐渐下降,14d仍有表达.结论 大鼠脑挫伤修复过程中MMP-3的表达呈时序性变化规律,可望用于脑挫伤时间的推断.
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| 关 键 词： | 法医病理学 脑损伤 基质金属蛋白酶3 免疫组织化学 印迹法,蛋白质 损伤时间推断 大鼠 |
Expression of Matrix Metalloproteinase-3 after Brain Contusion in Rats |
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| GUO Xiao-chong,LI Ru-bo,LIANG Hong-xia,WANG Fu-yuan,WU Xu. Expression of Matrix Metalloproteinase-3 after Brain Contusion in Rats[J]. Journal of Forensic Medicine, 2009, 25(1): 1-5. DOI: 10.3969/j.issn.1004-5619.2009.01.001 |
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| Authors: | GUO Xiao-chong LI Ru-bo LIANG Hong-xia WANG Fu-yuan WU Xu |
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| Affiliation: | GUO Xiao-chong1,2,LI Ru-bo1,LIANG Hong-xia1,WANG Fu-yuan1,WU Xu1 (1. Department of Forensic Pathology,School of Forensic Medicine,China Medical University,Shenyang 110001,China,2. Key Lab of Laboratory Transgenic Animal of Liaoning Province,Department of Labora- tory Animal Science,China) |
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| Abstract: | Objective To investigate the expression of matrix metalloproteinase-3 after brain contusion and its applicability for estimating the age of brain contusion. Methods Rats had been divided into three groups: control group, sham operation group and brain contusion group. The expression of matrix metallo- proteinase-3 at different time was detected by immunohistochemistry and Western blot. Results By the immunohistochemistry, no staining was observed in control and sham operation groups. The positive stain- ing... |
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| Keywords: | forensic pathology brain injuries matrix metalloproteinase-3 immunohistochemistry blotting western wound age estimation rats |
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