| Dot—ELISA在用酶标单克隆抗体检验体液斑和血痕ABH血型物质中的应用研究 |
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| 引用本文： | 王香菊,蒯应松,徐秀兰,温佩忠. Dot—ELISA在用酶标单克隆抗体检验体液斑和血痕ABH血型物质中的应用研究[J]. 中国法医学杂志, 1992, 0(3) |
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| 作者姓名： | 王香菊 蒯应松 徐秀兰 温佩忠 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所 北京 100038，北京 100038，北京 100038，北京 100038 |
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| 摘 要： | 本文应用Dot-ELISA法用酶标单克隆抗体,直接检验出稀释5万倍的精斑、10万倍的唾液斑及阴道分泌物等体液斑和血痕中ABH物质,还可检验出1～17年的体液斑中ABH物质。全部操作在室温中进行,以肉眼观察颜色变化判断结果,试验周期30～60分钟,准确率100％,实验结果可长期保存。
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| 关 键 词： | Dot-ELISA 体液斑 血痕 酶标单克隆抗体 ABH物质 |
APPLICATION OF DOT-ELISA WITH MONOCLONAL ANTIBODY FOR DETECTION ABH SUBSTANCES IN STAINS OF BODY FIUID AND BLOOD |
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| Wang Xiangju Kuai Yingsong Xu Xiulan Wen Peizhong Institute of Forensic. Ministry of Public Security,Beijing. APPLICATION OF DOT-ELISA WITH MONOCLONAL ANTIBODY FOR DETECTION ABH SUBSTANCES IN STAINS OF BODY FIUID AND BLOOD[J]. Chinese Journal of Forensic Medicine, 1992, 0(3) |
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| Authors: | Wang Xiangju Kuai Yingsong Xu Xiulan Wen Peizhong Institute of Forensic. Ministry of Public Security Beijing |
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| Affiliation: | Wang Xiangju Kuai Yingsong Xu Xiulan Wen Peizhong Institute of Forensic. Ministry of Public Security,Beijing 100038 |
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| Abstract: | The Monoclonal Antibodies linked with peroxidase was used in Dot-ELISA for detecting ABH substances in stains of body fluid and blood. Positive reacions were found in 5×10~4-fold dilution of seminal stains and 1×l0~4-fold dilution of salive stains and sweet stains and blood stains, ABH substances in 10-20 years old stains of body fluid were also detected. The positive rate was 100%. The test was performed in room tempreture. The test results showed that the direct Dot-ELISA with Monoclonal Antibodies was sensitive and specific enough for detecting the blooc group substances. The method is simple and easy. So it can be used in forensic science laboratory. |
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| Keywords: | Dot-ELISA Body fluid stains Blood stains The monoclonal antibody linked with peroxidase ABH |
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