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| 猪圆环病毒2型套式PCR检测方法的建立及应用 | |
| 引用本文： | 王贵平,蒋智勇,宋长绪,高向阳,赵亚华,林志雄,朱道中. 猪圆环病毒2型套式PCR检测方法的建立及应用[J]. 中国兽医科学, 2004, 34(10): 26-28 |
| 作者姓名： | 王贵平 蒋智勇 宋长绪 高向阳 赵亚华 林志雄 朱道中 |
| 作者单位： | 1. 广东省农业科学院,兽医研究所,广东,广州,510640 2. 广东省农业科学院,兽医研究所,广东,广州,510640;华南农业大学,生命科学院,广东,广州,510642 3. 华南农业大学,生命科学院,广东,广州,510642 4. 广州市出入境检验检疫局,广东,广州,510623 |
| 基金项目： | 国家高技术发展计划 (86 3)项目 (2 0 0 3AA2 4 1110 )，广东省农业攻关项目 (2 0 0 3B2 14 0 4 ) |
| 摘 要： | 根据GenBank登录的猪圆环病毒 2型 (PCV2 )的全基因组序列 ,设计了 2对引物 ,建立了检测PCV2的套式PCR方法。对该方法用序列分析、酶切等方法进行了鉴定。同时用该套式PCR方法对华南地区 2 87个猪场的 15 6 0份样品进行了检测 ,结果 ,983份为PCV2阳性 ,阳性率为6 3%。 |
| 关 键 词： | 猪圆环病毒2型 套式PCR 流行病学调查 |
| 文章编号： | 1000-6419(2004)10-0026-03 |
| 修稿时间： | 2004-06-28 |
Development of nested PCR for detection of PCV2 and its application to PCV2 epidemic investigation in South China |
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| WANG Gui-ping,JIANG Zhi-yong. Development of nested PCR for detection of PCV2 and its application to PCV2 epidemic investigation in South China[J]. Chinese Veterinary Science, 2004, 34(10): 26-28 | |
| Authors: | WANG Gui-ping JIANG Zhi-yong |
| Affiliation: | WANG Gui-ping~1,JIANG Zhi-yong~ |
| Abstract: | According to the published genome of porcine circovirus type 1 and type 2(PCV1,PCV2) two pairs of primers were designed for detecting PCV2 in samples by nested PCR assay. The nested PCR assay was identified by different enzyme restrictions and sequencing analysis. With this PCR method, 1 560 samples from 287 pig farms have been detected for PCV2, 983 samples of which got the positive results for PCV2. It was concluded from the (above-mentioned) result that PCV2 infection is very popular in South China. |
| Keywords: | porcine circovirus type 2 (PCV2) nested PCR epidemic investigation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |