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| 兔急性肺损伤后肺内VEGF时序性表达的研究 | |
| 引用本文： | 姚思佳,熊平,李焕铮,巫智勇,张静. 兔急性肺损伤后肺内VEGF时序性表达的研究[J]. 中国法医学杂志, 2009, 24(3) |
| 作者姓名： | 姚思佳 熊平 李焕铮 巫智勇 张静 |
| 作者单位： | 1. 南华大学法医病理学教研室,湖南,衡阳,421001 2. 南华大学生物化学与生物技术系,湖南,衡阳,421001 3. 湖南省第二人民医院,湖南,长沙,410007 |
| 摘 要： | 目的观察大潮气量机械通气致兔急性肺损伤(acutelung injury,ALI)后,肺组织中血管内皮生长因子(VEGF)的动态变化,探讨其在损伤时间推断中应用的价值。方法57只雄性新西兰大白兔随机分为正常组(3只)、对照组(27只)和实验组(27只)。机械通气(V_T 60mL/kg)致AL后于0,1,3,6,12,18,24,36,48h各处死动物3只。取肺组织,测量右上肺组织湿干重比(W/D),肺组织进行HE及免疫组织化学染色并进行图像定量分析,观察VEGF变化规律。结果正常组VEGF有低水平表达;大潮气量机械通气1min后至肺损伤后12h,实验组VEGF表达水平与正常组无明显差异(P>0.05);12～48h随损伤时间的延长VEGF表达水平持续上升,48h仍处于较高水平,其间各时间点VEGF表达水平与正常组比较均有显著性差异(P<0.05)。结论大潮气量机械通气致兔ALI 2d内,VEGF在肺组织炎症发生、发展和愈合修复过程中存在时间相关性,对法医病理推断呼吸机所致的ALI时间具有参考价值。 |
| 关 键 词： | 法医病理学 损伤时间 急性肺损伤 血管内皮生长因子 |
Study on the time-dependent expression of VEGF after acute lung injury in rabbits |
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| YAO Sijia,XIONG Ping,LI Huanzheng,WU Zhiyong,ZHANG Jing. Study on the time-dependent expression of VEGF after acute lung injury in rabbits[J]. Chinese Journal of Forensic Medicine, 2009, 24(3) | |
| Authors: | YAO Sijia XIONG Ping LI Huanzheng WU Zhiyong ZHANG Jing |
| Affiliation: | YAO Sijia XIONG Ping~1,LI Huanzheng~2,WU Zhiyong~3,ZHANG Jing~1/1.Department of Forensic Pathology,University of South China,Hunan Hengyang 421001,China,2.Faculty of Biochemistry and Biotechnology,3.Second People's Hospital of Hunan Province,Changsha 410007 |
| Abstract: | Objective To observe the dynamic change of vascular endothelial growth factor(VEGF) after acute lung injury(ALI) induced by high-V_T mechanical ventilation in rabbits,and explore the applicability to estimating wound age.Methods 57 healthy male white rabbits were randomly divided into 3 groups:normal group(n=3),control group(n=27),and trial group(n=27).Three animals were sacrificed at each time interval(0,1,3,6,12,18,24,36,48h) after ALI,respectively.After the lungs were removed,the wet/dry ratio of the upp... |
| Keywords: | forensic pathology wound age acute lung injury VEGF |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |