| 用地高辛配基标记的pAW101探针杂交分析DNA多态性及其应用 |
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| 引用本文： | 罗超权,杨英浩,伍新尧,罗健扬,刘超. 用地高辛配基标记的pAW101探针杂交分析DNA多态性及其应用[J]. 中国法医学杂志, 1994, 0(3) |
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| 作者姓名： | 罗超权 杨英浩 伍新尧 罗健扬 刘超 |
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| 作者单位： | 广州中山医科大学!广州，510089，广州中山医科大学!广州，510089，广州中山医科大学!广州，510089，广州中山医科大学!广州，510089，广州市刑事科学技术研究所!5130300 |
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| 摘 要： | 将NaCl盐析法抽提人基因组DNA与地高辛配基标记DNA探针的方法相结合，检测了15个家系子代与亲代DNApAW101－EcoRI限制性片段的遗传关系。结合所测同工酶（EsD．GLOI．pGM；．Acp）的基因频率、按Essen－moller氏公式计算，父权概率均达到99．73％以上。本法经济、有效、实用、易于在国内普遍实验室开展。
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| 关 键 词： | 限制性片段长度多态性 地高辛配基 亲权鉴定 |
RFLPs ANALYSIS OF DIGOXIGENIN-LABELLED pAW101 PROBE AND ITS PRACTICAL USES |
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| Liu Chao ,Luo Chaoquan ,Yang Yinghao,Wu Xinyao,Luo Jianyang. RFLPs ANALYSIS OF DIGOXIGENIN-LABELLED pAW101 PROBE AND ITS PRACTICAL USES[J]. Chinese Journal of Forensic Medicine, 1994, 0(3) |
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| Authors: | Liu Chao Luo Chaoquan Yang Yinghao Wu Xinyao Luo Jianyang |
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| Affiliation: | Liu Chao ;Luo Chaoquan ;Yang Yinghao;Wu Xinyao;Luo Jianyang(Sun yatsen University of Medical Sciences (Guangzhou 510089)) |
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| Abstract: | A new method to revel RFLPs is presented. The human genomic DNAwas purified by saturatedNaCl solution and the pAW101 probe labelled with digoxigenin-dUTP. The relationships of RFLPsand genetic patterns of PGM1 (phosphoglucomutase),EsD (esterase D),GLO1 (glyoxalase)and ACP(acid phosphatase ) between the fillal generation and parental generation were detected in 15 families(among them 11 cases were aborted fetuses). The probability of paternity (w)was caculated accor-ding to Essen - Moller's formula, each w vlua was over 99. 73 %, reached the standard of incladingpaternity. An effective,rapid, and non-toxic RFLPs technique was established, which is easy to man-age in common lab oratories. |
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| Keywords: | Restrtion fragment length polymorphisms (RFLPs) Digoxigenin Paternity determination |
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