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Fas在内毒素致大鼠肝细胞凋亡中的作用
引用本文:严玉霖,高洪,翁银标,陈玲,高利波,赵汝. Fas在内毒素致大鼠肝细胞凋亡中的作用[J]. 中国兽医科学, 2010, 40(1)
作者姓名:严玉霖  高洪  翁银标  陈玲  高利波  赵汝
作者单位:云南农业大学动物科学技术学院,云南,昆明,650201 
基金项目:国家自然科学基金项目,云南省自然科学基金项目 
摘    要:为探讨Fas在内毒素(ET)致大鼠肝细胞凋亡中的作用,将48只SD大鼠随机分为2组,1组为对照组(静脉注射生理盐水),2组为内毒素组(静脉注射内毒素5mg/kg)。分别在注射后第3、4、8、12h每组各剖杀6只大鼠,采取肝,用流式细胞术检测肝细胞凋亡情况,用免疫组织化学技术观察内毒素对肝细胞Fas表达的影响。结果显示,2组的肝细胞凋亡率明显高于1组(P0.01),且随着时间的延长呈上升趋势;2组的Fas积分光密度明显高于1组(P0.01),且一直呈上升趋势。结果表明,ET通过上调肝细胞中Fas的表达,可显著介导肝细胞的凋亡。

关 键 词:内毒素  肝细胞  细胞凋亡

Effect of Fas on hepatocyte apoptosis in rats induced by endotoxin
YAN Yu-lin,GAO Hong,WENG Yin-biao,CHEN Ling,GAO Li-bo,ZHAO Ru. Effect of Fas on hepatocyte apoptosis in rats induced by endotoxin[J]. Chinese Veterinary Science, 2010, 40(1)
Authors:YAN Yu-lin  GAO Hong  WENG Yin-biao  CHEN Ling  GAO Li-bo  ZHAO Ru
Affiliation:YAN Yu-lin,GAO Hong,WENG Yin-biao,CHEN Ling,GAO Li-bo,ZHAO Ru(College of Animal Science , Technology,Yunnan Agricultural University,Kunming 650201,China)
Abstract:Forty-eight SD rats were randomly divided into two groups of the normal control group ino-culated with normal saline by intravenous injection and the endotoxin(ET) treatment group inoculated with ET at 5mg/kg by intravenous injection to explore the effect of Fas on ET-induced hepatocyte apoptosis in rats.The liver samples were collected from 6 rats in each group at hour 3,4,8 and 12 post-injection,respectively.The apoptotic hepatocytes from the liver samples were examined by flow cytometry,and the effect of...
Keywords:Fas  endotoxin  hepatocyte  apoptosis  Fas
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