| 产蛋下降综合征病毒PCR检测方法的建立 |
| |
| 引用本文： | 刘大程,周伟光,关平原,张孝荣. 产蛋下降综合征病毒PCR检测方法的建立[J]. 中国兽医科学, 2004, 34(11): 56-59 |
| |
| 作者姓名： | 刘大程 周伟光 关平原 张孝荣 |
| |
| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018
2. 包头市农牧业局,内蒙古,包头014030 |
| |
| 基金项目： | 内蒙古自然科学基金资助项目 ( 2 0 0 10 90 4 0 6 ) |
| |
| 摘 要： | 根据已报道的腺病毒DNA序列 ,设计合成了 1对核苷酸引物 ,引物间隔约 63 0bp。用该引物对EDS76病毒内蒙古分离株 (N3 ,N4 )和参考株 (AV 1 2 7)核酸进行聚合酶链式反应 (PCR)。结果 ,均扩增出了约 63 0bp的特异性DNA片段。所建立的PCR方法可检出 1 0 0pg的样品模板。结果表明 ,此PCR方法是检测EDS76病毒的一种简便快速、特异性强和灵敏度高的方法
|
| 关 键 词： | 产蛋下降综合征病毒 PCR 检测 |
| 文章编号： | 1000-6419(2004)11-0056-04 |
| 修稿时间： | 2004-07-20 |
Development of PCR method for detection of egg drop syndrome virus |
| |
| LIU Da-cheng,ZHOU Wei-guang,GUAN Ping-yuan,ZHANG Xiao-rong. Development of PCR method for detection of egg drop syndrome virus[J]. Chinese Veterinary Science, 2004, 34(11): 56-59 |
| |
| Authors: | LIU Da-cheng ZHOU Wei-guang GUAN Ping-yuan ZHANG Xiao-rong |
| |
| Affiliation: | LIU Da-cheng~1,ZHOU Wei-guang~1,GUAN Ping-yuan~1,ZHANG Xiao-rong~2 |
| |
| Abstract: | One pair of primers were designded and synthesized according to the published DNA sequences of adenovirus. Using the Primers,a 630 bp DNA fragment was amplified from Nei Monggol strains N3,N4 and the standard strain AV-127 of EDS76 virus by polymerase chain reaction(PCR). A PCR method for detection of EDS76 virus was developed finally and it could detect 100 pg template. The results showed that the PCR method is a simple,quick,specific and sensitive method for detection of EDS76 virus. |
| |
| Keywords: | egg drop syndrome virus PCR detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
