The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces |
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| Authors: | Li‐Chin Tsai Ph.D. Cheng‐Chang Lee M.S. Chun‐Chieh Chen M.S. James Chun‐I Lee Ph.D. Sheng‐Meng Wang Ph.D. Nu‐En Huang Ph.D. Adrian Linacre Ph.D. Hsing‐Mei Hsieh Ph.D. |
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| Affiliation: | 1. Department of Forensic Science, Central Police University, Taoyuan, Taiwan;2. Forensic Science Center, New Taipei City Police Department, New Taipei City, Taiwan;3. Forensic Science Section, Changhua County Police Bureau, Changhua City, Taiwan;4. Department of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan;5. School of Biological Sciences, Flinders University, Adelaide, SA, Australia |
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| Abstract: | Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real‐time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2‐indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2‐indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2‐indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces. |
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| Keywords: | forensic science DNA typing fingerprint enhancement epithelial cells porous surfaces stochastic effects |
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