Age Estimation in Living Egyptians Using Signal Joint T‐cell Receptor Excision Circle Rearrangement |
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| Authors: | Samah F Ibrahim MD Iman F Gaballah MD Laila A Rashed MD |
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| Institution: | 1. Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo, Egypt;2. Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt |
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| Abstract: | Age estimation is one of the challenges in forensic sciences. There are many techniques to estimate the age. Molecular biology approach is one of these techniques. Signal joint T‐cell receptor excision circles gene (sjTRECs), is one of this approach. We aimed to use sjTRECs as a suitable marker for age estimation among Egyptian population. TaqMan qPCR approach was used to quantify sjTREC levels in blood samples obtained from 153 healthy Egyptian individuals ranging from a few weeks to 70 years. Our results showed a significant negative correlation between sjTREC levels and age with p ≤ 0.05. Moreover, the individual's age can be determined through this formula Age = ?30.671+ (?5.998Y) (Y is dCtTBP ? sjTREC) with standard error ±7.35 years. Within the forensic context, sjTREC' levels can be used to estimate the Egyptian individual's age accurately. |
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| Keywords: | forensic science DNA typing signal joint T‐cell receptor excision circles Egyptian age estimation polymerase chain reaction |
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