Cutaneous (tPA) and Skeletal (TnI) mRNA as Markers of Aging in Contused Wound |
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| Authors: | Samah F Ibrahim MD Marwa Issak MD Amany A Bayoumy MD Dina S Abd El‐Fatah MD |
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| Institution: | 1. Lecturer of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo, Egypt;2. Professor Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt |
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| Abstract: | Wound age estimation is one of the most important forensic aspects. Troponin I (TnI) and many cytokines, for example, tissue plasminogen activator (tPA), are involved in wound inflammation and healing. Skeletal (TnI) and cutaneous (tPA) mRNA was detected using real‐time PCR in 25 female albino rats. They were divided into 5 groups: control and 4 injured groups. Injured groups were sacrificed 1, 6, 24, and 30 h after inflicting contused wound. The expression levels of cutaneous (tPA) were decreased significantly at 1, 6, and 30 h after contusion (71.7%, 30.7 and 16.9%), while the expression levels of skeletal (TnI) were increased significantly at 1 and 6 h post‐traumatic, then they gradually decreased until reaching normal levels at 24 h and assumed significantly lower levels at 30 h postcontusion. These results suggested that the determination of cutaneous (tPA) and skeletal (TnI) mRNA levels was useful for wound age estimation. |
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| Keywords: | forensic science wound age estimation messenger RNA real‐time PCR contused wound blunt trauma |
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