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汗潜指印的STR分型检测在案件中的应用
作者姓名:Dong Y  Zhang C  Gu LH  Ping Y  Zheng HF
作者单位:上海市刑事科学技术研究所,上海,200083
摘    要:目的探索案例中所涉及的汗潜指印DNA的提取和检验方法。方法采用Chlex-100法提取DNA,进行STR复合扩增,通过毛细管电泳检测荧光信号。结果案例中涉及的汗潜指印在ProfilerPlus试剂盒10个基因座的分型检测均获成功。结论含有汗潜指印的检材的发现和正确提取对最终检测成功至关重要。

关 键 词:汗潜指印  DNA检验  STR分型
文章编号:1004-5619(2006)01-0074-02
修稿时间:2005年8月10日

PCR-based STR genotyping of sweat latent fingerprint samples involved in cases
Dong Y,Zhang C,Gu LH,Ping Y,Zheng HF.PCR-based STR genotyping of sweat latent fingerprint samples involved in cases[J].Journal of Forensic Medicine,2006,22(1):74-75.
Authors:Dong Yan  Zhang Cheng  Gu Li-hua  Ping Yuan  Zheng Hui-fen
Institution:Forensic Science and Technology Institute of Shanghai Public Security Bureau, Shanghai 200083, China.
Abstract:Objective To study the extracting and genotyping method of sweat latent fingerprint samples involved in cases. Method Chlex 100 extraction method was used to extract DNA. STR loci were typed after PCR amplification by Profiler Plus kit. Results All the sweat latent fingerprint samples involved in cases obtained reliable results of STR genotyping. Conclusion It is very important to find and extract sweat latent fingerprint samples properly for STR genotyping.
Keywords:sweat latent fingerprints  DNA examination  STR genotyping
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