| 采用SNP分型方法对甲醛固定组织进行个体识别 |
| |
| 作者姓名： | Lin Y Li L Liu Y Zhao ZM |
| |
| 作者单位： | 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063 |
| |
| 基金项目： | 中央级科研院所社会公益研究专项项目(GY0803) |
| |
| 摘 要： | 目的通过对X染色体SNP遗传标记的检测,探讨甲醛固定组织块的DNA分型策略。方法提取甲醛固定组织块的DNA,在用SinofilerTM试剂盒、MiniFilerTM试剂盒检测未能获得分型结果的情形下,采用多重PCR和飞行质谱技术对X染色体上的51个SNP位点进行分型检测。结果对于常染色体STR、miniSTR分型失败的甲醛固定组织块,X-SNP分型获得成功。结论对于甲醛固定组织块等微量、降解的生物学检材,若常染色体STR基因座分型失败,可尝试进行SNP位点的分型,以获得更多的遗传信息。
|
| 关 键 词： | 法医遗传学 多态性 单核苷酸 甲醛 个体识别 |
SNP typing of formalin-fixed tissue in individual identification |
| |
| Lin Y,Li L,Liu Y,Zhao ZM.SNP typing of formalin-fixed tissue in individual identification[J].Journal of Forensic Medicine,2010,26(6):446-448. |
| |
| Authors: | Lin Yuan Li Li Liu Yan Zhao Zhen-min |
| |
| Institution: | LIN Yuan,LI Li,LIU Yan,ZHAO Zhen-min(Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China) |
| |
| Abstract: | Objective To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.Methods Genomic DNA was prepared from formalin-fixed tissue.In the event that typing using SinofilerTM and MiniFilerTM kits had failed,51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS).Results Full profiles of X-SNP loci were obtained from the formalin-fixed tissue,while the STR and miniSTR genotypin... |
| |
| Keywords: | forensic genetics polymorphism single nucleotide formaldehyde individual identification |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
