JL-02多位点探针DNA指纹的法医学应用研究 |
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引用本文: | 季安全,李继周,叶健,倪锦堂,李伯龄. JL-02多位点探针DNA指纹的法医学应用研究[J]. 中国法医学杂志, 1997, 0(2) |
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作者姓名: | 季安全 李继周 叶健 倪锦堂 李伯龄 |
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作者单位: | 北京公安部第二研究所!北京,100038,北京公安部第二研究所!北京,100038,北京公安部第二研究所!北京,100038,北京公安部第二研究所!北京,100038,北京公安部第二研究所!北京,100038 |
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摘 要: | 以自制的JL-02探针进行了DNA指纹分析,对北京地区无关个体进行了调查,计算出任意两无关个体的偶合机率为6.6×10-15;家系分析表明,谱带在亲代与子代间的传递符合孟德尔遗传规律;同一个体不同组织的DNA指纹图相同;混合斑精子DNA指纹图与相应男性血液DNA指纹图完全相同;该探针对0.5μg的基因组DNA杂交,可获得清晰可辩的DNA指纹图。证明了新探针适用于法医物证检验中的个人同一认定及亲子鉴定。
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关 键 词: | DNA指纹 多位点探针 个人同一认定 亲子鉴定 |
APPLICATION OF JL-02 PROBE FINGERPRINTS IN FORENSIC SCIENCE |
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Abstract: | A new version of multilocus probe (JL-02)was developed to detect human genomic DNA fingerprints.The resulting DNA fingerprints were highly polymorphic.To determine the variability of JL-02 fingerprints,DNA samples form a panel of 25 unrelated chinese on the same membrande were fingerprinted using probe JL-02.Pairwise comparisons of the DNA fingerprints showed that the probability of two unrelated indiveduals having identical profiles is 6.6×10-15.Pedigree analysis showed that these polymorphic fragments were stably inherited and segregate in a Mendelian fashion.In addition,DNA fingerprints obtained form diffirent tissues of the same individual were identical .These results comfirmed that JL-02 fingerprints were germ-line and somatic stable .Our preliminary results demonstrate that JL-02fingerprints are suitable are suitable for forensic case work. |
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Keywords: | DNA fingerprints Multilocus JL-02 probe Individual indentification Paternity testing |
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