头发中5-MeO-DiPT等12种新型色胺类致幻剂的UPLC-MS/MS分析方法研究

目的建立同时分析头发中5-MeO-DiPT等12种新型色胺类致幻剂的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。方法以赛洛西宾-d4和赛洛新-d10为内标,20 mg头发样品加入1 mL提取液(含内标2 ng/mL的0.1%甲酸水溶液)后冷冻研磨,离心取上清液,经Waters Acquity^TM UPLC HSS T3色谱柱分离,采用多反应监测模式同时测定12种新型色胺类致幻剂。结果头发样品中12种色胺类新精神活性物质的检出限为1~10pg/mg,定量限为3~50pg/mg。在相应浓度范围内,12种色胺类新精神活性物质均具有良好的线性关系,相关系数大于0.99。本方法准确度为91.3%~113.5%,日间和日内精密度(RSD)均小于15%,回收率大于80%,无明显基质效应。结论该方法前处理简单、灵敏度高、选择性好,适用于法医毒物分析中新型色胺类致幻剂的鉴定。

Research on the Analytical Method of New Tryptamine Hallucinogens Such as 5-Meo-DiPT in Hair Samples by UPLC-MS/MS

Objective To development a method for simultaneous determination of 12 tryptamine hallucinogens in hair samples by UPLC-MS/MS.Methods With Psilocybin-d4 and Psilocin-d10 as internal standards,20 mg hair sample was frozen and ground after adding 1 ml extract,and centrifuged to obtain the supernatant.The supernatant was then separated in a waters AcquityTM UPLC HSS T3 column with a gradient elution,finally analyzed with multiple reaction monitoring(MRM)mode.Results The limits of detection(LOD)of 12 tryptamine-type new psychoactive substances in hair samples are ranging from 1-10 pg/mg,the lower limits of quantitation(LLOQ)are ranging of 3-50 pg/mg.In the corresponding concentration range,there was a good linearity for all analytes with correlation coefficients over 0.99.The accuracy was 91.3%-113.5%,the intra-day and inter-day precision were less than 15%,and the extraction recovery of all analytes were above 80%.Besides,no significant matrix effect was exhibited.Conclusion The established method is rapid,sensitive and specific,and it be used for the authentic cases of forensic toxicology.