| 1066例新疆汉族人群21个常染色体STR基因座遗传多态性分析 |
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| 引用本文： | 李亮亮,陈晓鸣,李学博,张建中.1066例新疆汉族人群21个常染色体STR基因座遗传多态性分析[J].中国司法鉴定,2020(2):37-41. |
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| 作者姓名： | 李亮亮 陈晓鸣 李学博 张建中 |
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| 作者单位： | 山东政法学院山东省高校证据鉴识重点实验室;山东政法学院司法鉴定中心;常山县公安局;阿勒泰地区公安局 |
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| 基金项目： | 山东省高校科技计划项目(J17KA240);湖北崇新司法鉴定中心&刘良工作室开放课题(CXLL201803);山东省高校证据鉴识重点实验室(山东政法学院)开放课题(2018KFKT7);四川医事卫生法治研究中心资助课题(YF19-Q16)。 |
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| 摘 要： | 目的研究21个常染色体STR基因座(CSF1PO,D3S1358,D5S818,D7S820,D8S1179,D13S317,D16S539,D18S51,D21S11,FGA,TH01,VWA,D2S1338,D19S433,D1S1656,D12S391,D2S441,D10S1248,TPOX,D22S1045,SE33)在新疆汉族人群中的遗传多态性。方法用GlobalFiler^TM R PCR Amplification荧光标记试剂盒对1066例新疆汉族无关个体的DNA进行PCR扩增,3500遗传分析仪电泳分析,用GeneMapper■ID-X v1.4软件分析等位基因片段大小,用Modified-Powerstates和Arlequin v3.5分析软件进行等位基因频率和法医学常用参数统计分析。结果在新疆汉族人群中,21个常染色体STR基因座不存在连锁不平衡现象,基因型分布符合Hardy–Weinberg平衡,共检出282个等位基因和1147种基因型,杂合度期望值(He)范围从0.6291(TPOX)到0.9428(SE33),多态信息含量(PIC)范围从0.5648(TPOX)到0.9393(SE33),累计个体识别率(CDP)>0.99999999999999999999。结论新疆汉族人群21个常染色体STR基因座具有较高多态性,可以用于法医学亲权鉴定和个体识别,也可以用于人类学和遗传学研究。
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| 关 键 词： | 法医遗传学 遗传多态性 STR基因座 连锁不平衡 |
Analysis of Genetic Polymorphisms of 21 Autosomal STR Loci in 1066 Individuals from the Han Ethnic Group from Xinjiang Province |
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| LI Liangliang,CHEN Xiaoming,LI Xuebo,ZHANG Jianzhong.Analysis of Genetic Polymorphisms of 21 Autosomal STR Loci in 1066 Individuals from the Han Ethnic Group from Xinjiang Province[J].Chinese Journal of Forensic Sciences,2020(2):37-41. |
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| Authors: | LI Liangliang CHEN Xiaoming LI Xuebo ZHANG Jianzhong |
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| Institution: | (Key Laboratory of Evidence Identification in Universities of Shandong Province,Shandong University of Political Science and Law,Jinan 250014,China;The Center of Judicial Identification of Shandong University of Political Science and Law,Jinan 250014,China;Changshan Public Security Bureau,Changshan 324200,China;Altay Public Security Bureau of Xinjiang Uygur Autonomous Region,Altay 836500,China) |
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| Abstract: | Objective To analyze the genetic polymorphism from the Han Ethnic Group from Xinjiang of 21 autosomal STR loci, including CSF1 PO, D3 S1358, D5 S818, D7 S820, D8 S1179, D13 S317, D16 S539, D18 S51, D21 S11,FGA, TH01, VWA, D2 S1338, D19 S433, D1 S1656, D12 S391, D2 S441, D10 S1248, TPOX, D22 S1045, and SE33 and to provide genetic data for the forensic application of individual and paternity identification. Method Amplification of 21 autosomal STR loci was performed using the GlobalFilerTM kit. PCR products were separated and detected by capillary electrophoresis in an ABI 3500 Genetic Analyzer.Genotyping was done using allelic ladders provided with the kit and the GeneMapper? ID-X software v1.4.Results No significant deviations from the Hardy–Weinberg equilibrium and linkage disequilibrium were detected from the Han Ethnic Group from Xinjiang, 282 alleles and 1 147 genotypes were gained. The expected heterozygosity(He) of the population ranged from 0.6291(TPOX) to 0.9428(SE33) while Polymorphism Information Content(PIC) ranged from 0.5648(TPOX) to 0.9393(SE33).The combined power of discrimination of the population is greater than 0.99999999999999999999. Conclusion The 21 autosomal STR loci of Han Ethnic Group from Xinjiang have relatively high genetic polymorphism and can provide reference for researches in the field of ethnical genetics and forensic genetics. |
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| Keywords: | forensic genetics genetic polymorphism STR loci linkage disequilibrium |
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