傅里叶红外光谱技术在法医学脑挫裂伤鉴定中的应用展望

脑挫裂伤是常见的一类颅脑损伤,单纯的组织病理学技术可能难以早期发现及精确诊断。傅里叶变换红外面扫描成像技术(FTIR-mapping)是病理学研究领域的新技术,因其结合了傅里叶变换红外光谱、红外显微镜技术以及面扫描成像技术的特点,具有测定方法简便、对样本无损、灵敏度高、准确、直观等优点,突破了传统技术的局限,能检测出发生了物质数量、结构以及构象变化但缺乏明显形态学变化的脑挫裂伤,从而实现早期精准地诊断脑挫裂伤。FTIR-mapping虽已能绘制某些损伤组织的红外光谱病理图像,但其在法医病理学中的价值有待全面深入开发。本文综述了脑挫裂伤的法医病理学研究进展以及傅里叶变换红外面扫描成像技术在法医病理学中的应用。

Insight on Applications of Fourier Transform Infrared Spectroscopy into Forensic Identifi cation of Cerebral Contusion or Laceration

Cerebral contusion/laceration is one commonly-seen craniocerebral injury.Single histopathological techniques are diffi cult to early detect or accurately diagnose cerebral contusion/laceration.Fourier transform infrared spectrum mapping(FTIR-mapping),a new approach for pathology,integrates the specifi cs of Fourier transform infrared spectroscopy,infrared microscopy and mapping,therefore making it assume the advantages of simple measuring,no damaging to sample,high sensitivity,accuracy and convenience for observation.FTIR-mapping breaks through the limitations of traditional technology,with the ability to detect the cerebral contusion/laceration that has changed in quantity,structure and conformation yet lacked obvious morphological alterations,thus realizing the early and accurate diagnosis of cerebral contusion/laceration.Although FTIR-mapping has presently been able to depict the infrared spectral pathologic images of some damaged tissues,its potential in forensic pathology is still expecting to be thoroughly developed.This article reviews the progress of forensic pathological studies on cerebral contusion/laceration,with inclusion of the applications of FTIR-mapping into forensic pathology.