甲状腺滤泡上皮细胞DNA含量与死亡时间的相关性

目的探讨甲状腺滤泡上皮细胞平均DNA含量变化与死亡时间(PMI)的相关性。方法采用甲基绿-派洛宁(MG-P)染色法结合图像分析技术,测定尸体甲状腺滤泡上皮细胞平均DNA含量变化值,并对其数据进行统计学分析。结果甲状腺滤泡上皮细胞平均DNA含量随死后经过时间的延长而加速降解。平均DNA含量各指标(平均灰度、目标面积、目标面积比)与PMI之间的决定系数R2分别为0.960、0.987、0.988和0.990(P<0.001)。结论尸体甲状腺滤泡上皮细胞平均DNA含量与PMI之间具有明显相关性,甲基绿-派洛宁(MG-P)染色法结合图像分析技术测定在研究甲状腺滤泡上皮细胞DNA含量变化方面具有一定优势。

The correlation between the DNA content in human thyroid follicular epithelial cells and the postmortem interval

Objective To study the relationship between the DNA content in follicular epithelial cells of the human thyroid and postmortem interval(PMI) .Methods Changes of the DNA content in thyroid follicular epithelial cells at different PMI were determined by Methyl Green-Pyronin(MGP) stain combined with an image analysis technique.Results The average DNA content in the thyroid follicular epithelial continued to decrease with increased PMI.The coefficient of determination for DNA content with average gray,aimed area,aimed area ratio and positive index in follicular epithelial cells were 0.960,0.987,0.988 and 0.990,respectively.Conclusion There is an apparent correlation between the average DNA content of follicular epithelial cells and the PMI.MGP stain combined with an image analysis technique seems to be a useful means to study the degradation of DNA in thyroid follicular epithelial cells.