Identification of human skin from a tissue fragment by detection of squamous cell carcinoma-related antigen using an enzyme immunoassay |
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| Affiliation: | 1. Postgraduate Program in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Federal University of Espírito Santo (UFES), Av. Marechal Campos, 1468, Maruípe, Vitória, ES, Brazil;2. Laboratory of Forensic Toxicology, State Policy, Vitória, Espírito Santo, Brazil;1. Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo;2. Department of Thoracic Oncology, Saitama Cancer Center, Kita-adachi-gun;3. Division of Integrated Oncology, Institute of Biomedical Research and Innovation, Kobe;4. Department of Thoracic Medical Oncology, Cancer Institute Hospital of JFCR, Tokyo;5. Clinical Research, Innovation and Education Center, Tohoku University Hospital, Sendai;6. Department of Respiratory Medicine and Medical Oncology, Yokohama Municipal Citizen''s HospitalHodogaya-ku;7. Department of Medical Oncology, KKR Sapporo Medical Center, Sapporo;8. Department of Medical Oncology, Japanese Red Cross Medical Center, Tokyo;9. Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba;10. Department of Respiratory Medicine, Saitama Medical University International Medical Center, Saitama;11. Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki;12. Division of Thoracic Oncology, National Cancer Center Hospital East, Kashiwa;13. Department of Pulmonary Medicine, Sendai Kousei Hospital, Sendai;14. Department of Respiratory Medicine, Gunma Prefectural Cancer Center, Ota;15. Department of Respiratory Medicine, National Hospital Organization Nagasaki Medical Center, Omura;16. Department of Internal Medicine, Niigata Cancer Center Hospital, Niigata;17. Department of Clinical Medicine (Biostatistics and Pharmaceutical Medicine), Kitasato University School of Pharmacy, Tokyo;18. Double-Barred Cross Hospital Japan Anti-Tuberculosis Association, Tokyo, Japan |
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| Abstract: | A new method of identifying human skin from a tissue fragment by detection of squamous cell carcinoma-related (SCC) antigen, using an enzyme immunoassay, was developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in extracts diluted 102-fold. There was no difference in the detection limit between individuals. Species specificity was good, and there was no cross reaction observed with skins from animals. Our method could also discriminate between skin and other organs or tissues, except for esophagus and lung. A practical case to which this method was applied is presented. |
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