Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging |
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| Authors: | Tiantong Yang M.D. Guanglong He M.D. Xiang Zhang M.D. Lin Chang M.D. Haidong Zhang M.D. Mary G. Ripple M.D. David R. Fowler M.D. Ling Li M.D. |
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| Affiliation: | 1. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, , Haidian District, Beijing, 100088 China;2. Institute of Forensic Science of Ministry of Public Security, , Xicheng District, Beijing, 100088 China;3. Departments of Pathology and Pediatrics, University of Maryland School of Medicine, , Baltimore, MD, 21201 |
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| Abstract: | The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β‐amyloid precursor protein (β–APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β‐APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques. |
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| Keywords: | forensic science forensic pathology diffuse axonal injury traumatic brain injury Fourier transform infrared spectroscopy animal study |
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