| 水牛卵母细胞去核方法的比较 |
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| 引用本文： | 陈自洪,杨素芳,谢英,李日聪,韦精卫,石德顺. 水牛卵母细胞去核方法的比较[J]. 中国兽医科学, 2006, 36(6): 493-496 |
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| 作者姓名： | 陈自洪 杨素芳 谢英 李日聪 韦精卫 石德顺 |
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| 作者单位： | 广西大学,动物繁殖研究所,广西,南宁,530005 |
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| 摘 要： | 水牛卵母细胞体外成熟22 h后,分别用盲吸法、点击法和纺锤体成像系统(Spindleview System)去核。结果,三者的去核率分别为65．1％、81．8％和95．0％,差异极显著(P< 0．01)。以水牛胎儿成纤维细胞为供体,通过核移植构建的重构胚的融合率、分裂率和囊胚发育率, 在上述3种方法之间均没有显著差异(P>0．05)。认为点击法是一种较为简单、实用和有效的去核方法。
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| 关 键 词： | 水牛 卵母细胞 去核 核移植 点击法 |
| 文章编号： | 1673-4696(2006)06-0493-04 |
| 修稿时间： | 2005-12-01 |
Comparison of enucleation protocols of buffalo oocytes |
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| CHEN Zi-hong,YANG Su-fang,XIE Ying,LI Ri-cong,WEI Jing-wei,SHI De-shun. Comparison of enucleation protocols of buffalo oocytes[J]. Chinese Veterinary Science, 2006, 36(6): 493-496 |
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| Authors: | CHEN Zi-hong YANG Su-fang XIE Ying LI Ri-cong WEI Jing-wei SHI De-shun |
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| Abstract: | Buffalo oocytes matured in vitro for 22 h were enucleated by blind-sucking, point-hitting and Spindle-View System, respectively. The enucleation rate of oocytes by the Spindle-View System (95%) was significantly(P<0. 01) higher than that achieved by blind-sucking(65.1%) and the pointhitting(81. 8%). However, there was no significant difference in fusion ration, cleavage rate and blasto cyst yield of the reconstructed embryos among the three enucleation methods(P>0.05). Results indicated that the point-hitting can be employed for enucleation of oocytes in consideration of the costs of the Spindle-View System. |
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| Keywords: | buffalo oocyte enucleation nuclear transplantation point-hitting |
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