SPE/UPLC法检测血中吗啡、苯丙胺类及氯胺酮

目的建立SPE/UPLC方法在同一条件下同时检测血中吗啡、苯丙胺类及氯胺酮。方法采用SCX 3cc（60mg）固相萃取柱萃取血中吗啡、MA、MDMA、MDA及氯胺酮,用超高效液相色谱（UPLC）-二极管阵列检测器（PDA）检测,结合保留时间和紫外光谱进行定性、定量分析,对实验各环节进行优化,并进行实际案例检测。结果吗啡、MA、MDMA、MDA、氯胺酮的固相萃取提取回收率分别为81.4%±2.51%、88.2%±2.48%、91.8%±2.03%、93.8%±1.46%、74.8%±2.27%,峰面积和质量浓度的线性关系良好（r〉0.999）,线性范围分别为0.08～100μg/mL、0.4～100μg/mL、0.2～75μg/mL、0.3～75μg/mL、0.4～100μg/mL,检出限分别为30pg、200pg、80pg、100pg、200pg。结论本文所建方法适用于血中吗啡、苯丙胺类、氯胺酮常见毒品的筛选及定量分析。

The simultaneous analysis of morphine,amphetamines and ketamine in whole blood by SPE/UPLC

Objective To establish a solid phase extraction(SPE)/UPLC method for the determination of morphine,amphetamines and ketamine in whole blood simultaneously.Methods Morphine,MA,MDMA,MDA,ketamine were extracted from whole blood using Agilent SCX 3cc(60mg)extraction cartridges and detected by UPLC-PDA.Qualitative and quantitative analysis was obtained by retention time and UV-spectrum.Results The recoveries for morphine,MA,MDMA,MDA,ketamine were 81.4%±2.51%,88.2%±2.48%,91.8%±2.03%,93.8%±1.46%,74.8%±2.27% respectively,The correlation coefficient of linear calibration curve was over 0.999 within concentration range 0.08～100μg/mL,0.4～100μg/mL,0.2～75μg/mL,0.3～75μg/mL,0.4～100μg/mL respectively.The limits of qualification were 30pg,200pg,80pg,100pg,200pg respectively.Conclusion The method was simple,rapid and accurate for qualitative and quantitative analysis of morphine,amphetamines,ketamine in whole blood simultaneously.