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| PCR-RFLP在mtDNA多态性分析中的应用 | |
| 引用本文： | 张明阳,单海燕,徐冬冬,丁梅,王保捷,庞灏,周勤虎,徐振亮. PCR-RFLP在mtDNA多态性分析中的应用[J]. 中国法医学杂志, 2011, 26(1): 48-49,53,89 |
| 作者姓名： | 张明阳 单海燕 徐冬冬 丁梅 王保捷 庞灏 周勤虎 徐振亮 |
| 作者单位： | 1. 南通大学医学院法医学教研室,江苏,南通,226000 2. 南通大学附属医院普外科,江苏南通,226000 3. 川北医学院法医系,四川,南充,640073 4. 中国医科大学法医学院法医血清教研室,辽宁,沈阳,110001 |
| 摘 要： | 目的探讨PCR-RFLP法在mtDNA多态性分析中的应用价值。方法应用限制酶RsaI和MnlI消化mtDNA D-LOOP区（HVI16106～16297）PCR扩增产物,聚丙烯凝胶电泳进行RFLP分型,对不同PCR-RFLP分型扩增产物进行测序,并对150例辽宁汉族随机个体及30例真三联家系血样进行检验。结果在150例随机个体中,RsaI和MnlI酶切分别发现3和8种表型,DNA测序结果和PAGE分型结果一致,GD值分别为0.107,0.670。联合两种酶切,发现12种表型,GD值为0.708。在30个家系中,母子的RFLP带型完全相同。结论 PCR-RFLP法适合在基层实验室mtDNA分析中应用。 |
| 关 键 词： | 法医物证学 多态性 PCR-RFLP技术 线粒体DNA |
The analysis of mtDNA polymorphisms using the PCR-RFLP technique |
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| ZHANG Mingyang,SHAN Haiyan,XU Dongdong,DING Mei,WANG Baojie,PANG Hao,ZHOU Qinhu,XU Zhenliang. The analysis of mtDNA polymorphisms using the PCR-RFLP technique[J]. Chinese Journal of Forensic Medicine, 2011, 26(1): 48-49,53,89 | |
| Authors: | ZHANG Mingyang SHAN Haiyan XU Dongdong DING Mei WANG Baojie PANG Hao ZHOU Qinhu XU Zhenliang |
| Affiliation: | (ZHANG Mingyang1,SHAN Haiyan2,XU Dongdong3,DING Mei4,WANG Baojie4,PANG Hao4 ZHOU Qinhu4,XU Zhenliang4 1.Department of Forensic Medicine,Nantong University,Jiangsu Nantong 226000,China,2.Department of General Surgery,Affiliated Hospital of Nantong University,3.Department of Forensic Science,North Sichuan Medical College,Sichuan Nanchong 640073,4.School of Forensic Medicine,China Medical University,Shenyang 110001,China) |
| Abstract: | Objective To study the application of the RFLP technique in mitochondrial DNA(mtDNA) analysis.Methods The hypervariable region 16106～16297nt in the D-LOOP of mtDNA was amplified by PCR,and then the fragments were digested by two restriction enzymes(RsaI,MnlI).150 unrelated Liaoning Han individuals and 30 case samples of true triple family were analyzed by this PCR-RFLP technique.In addition,different amplification products were sequenced.Results In 150 unrelated Liaoning Han individuals,three and eight genotypes were found by RsaI-RFLP and MnlI-RFLP analysis,and the gene diversity(GD) was estimated to be 0.107 and 0.670 respectively.Total 12 genotypes were detected using RsaI combined with MnlI,and the GD reached 0.708.In no instance,the mother displayed alleles different from those of the child within each trio in 30 family trios.Conclusion PCR-RFLP was a suitable technique for mtDNA analysis in general lab. |
| Keywords: | forensic biological evidence polymorphism PCR-RFLP technology mtDNA |
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