| 固相萃取/气相色谱法检测全血中的扎来普隆 |
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| 引用本文： | 张蕾萍,于忠山,何毅,王芳琳,常靖. 固相萃取/气相色谱法检测全血中的扎来普隆[J]. 中国法医学杂志, 2011, 26(1): 30-32 |
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| 作者姓名： | 张蕾萍 于忠山 何毅 王芳琳 常靖 |
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| 作者单位： | 公安部物证鉴定中心,北京,100038 |
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| 基金项目： | 中央公益性基金项目(编号2010JB001) |
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| 摘 要： | 目的建立全血中扎来普隆的固相萃取/气相色谱检测方法。方法采用Oasis HLB固相萃取柱,5%甲醇水淋洗,二氯甲烷洗脱,GC/NPD定量检验,GC/MS定性检验,并对检测条件进行考察。结果扎来普隆与血中杂质分离良好,平均萃取回收率达89%以上,最低检出限20ng/mL,50～2000ng/mL内线性良好,相关系数R2=0.9945,日内RSD为3.1%～4.2%,日间RSD 5.8%～6.7%。结论该方法操作简便快速,提取回收率高,重现性好,提取物杂质少,可用于实际案件的检验。
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| 关 键 词： | 法医毒物分析 扎来普隆 固相萃取 气相色谱 |
Determination of zaleplon in human blood by solid phase extraction-gas chromatography method |
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| ZHANG Leiping,YU Zhongshan,HE Yi,WANG Fanglin,CHANG Jing. Determination of zaleplon in human blood by solid phase extraction-gas chromatography method[J]. Chinese Journal of Forensic Medicine, 2011, 26(1): 30-32 |
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| Authors: | ZHANG Leiping YU Zhongshan HE Yi WANG Fanglin CHANG Jing |
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| Affiliation: | (ZHANG Leiping,YU Zhongshan,HE Yi,WANG Fanglin,CHANG Jing Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To develop a method of zaleplon blood by solid phase extraction-gas chromatography in human.Methods Purifying by using Oasis HLB solid phase extraction column,washing by 5% methanol-water and escaping by dichloromethane,quantitatively detecting with GC/NPD and qualitatively analyzing by GC/MS.Results Zaleplon and impurity in blood were separated well.The mean recovery rate of zaleplon in human blood was over 89%,the limits of detection was 20ng/mL.The linearity relationship was excellent between 5... |
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| Keywords: | forensic toxicological analysis zaleplon solid phase extraction gas chromatography |
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