实验性大鼠脑震荡后c-jun mRNA表达

目的观察实验性大鼠脑震荡后c-jun mRNA的表达变化规律。方法 55只实验大鼠随机分为脑震荡组（0min、15min、30min、60min、3h、6h、12h、24h、48h、96h）和对照组,用原位杂交法观察大鼠脑震荡后各时间点,大脑皮质、脑干和丘脑神经元c-jun mRNA表达的变化规律。结果对照组大鼠神经元和胶质细胞均可见c-jun mRNA的弱阳性表达。脑震荡组大鼠损伤后15min神经细胞观察到c-jun mRNA阳性表达,随损伤后经过时间的延长阳性表达逐渐增强;30min时c-jun mRNA阳性反应达高峰,随后逐渐降低,至96h时回落至对照组水平。结论 c-jun mRNA表达水平可成为诊断脑震荡和推断伤后经过时间的一项敏感指标。

A study on the expression of c-jun mRNA after experimental rat brain concussion

Objective To evaluate changes of c-jun mRNA after brain concussion.Methods Fifty-five rats were randomly divided into brain concussion groups(0min,15min,30min,60min,3h,6h,12h,24h,48h,96h) and control group.The expression of c-jun mRNA in cortex、thalamus and brain stem was microscopically observed by In site hybridization method.Results There were weak positive expression of c-jun mRNA in some neutrons and neuroglia cells in control group.In brain concussion group,however,positive expression of c-jun mRNA in some neutrons was seen at 15min after brain concussion,and reach to the peak at 30min after brain concussion the level of expression of c-jun mRNA were as well as control group at 96h.Conclusion There findings suggest that detection of c-jun mRNA could be an index of diagnosis of brain concussion and a sensitive marker of timing of injury after brain concussion.