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| 利用不同温度下人肝细胞DNA降解参数推断早期PMI | |
| 引用本文： | 李姗姗,赵春明,刘泉,刘良. 利用不同温度下人肝细胞DNA降解参数推断早期PMI[J]. 中国法医学杂志, 2011, 26(1): 4-6 |
| 作者姓名： | 李姗姗 赵春明 刘泉 刘良 |
| 作者单位： | 1. 齐齐哈尔医学院病理教研室,黑龙江,齐齐哈尔,161006 2. 湖北警官学院,湖北,武汉,430030 3. 中国政法大学证据科学教育部重点实验室,北京,100088 |
| 摘 要： | 目的对不同温度下人体肝细胞DNA降解状态进行多参数定量分析,得出温度相关的多元回归方程。方法选取13例已知PMI的人体离体肝脏,在死后13～34h内,分别在10℃、20℃及30℃条件下,每隔1h取材,进行细胞学涂片,Feulgen-Vans染色,选用图像分析系统检测几何参数（ID）及灰度参数（AOD）指标,对不同温度的检测指标进行多元回归分析,得出相应的回归方程。结果温度相关的肝细胞DNA降解推断死亡时间的回归方程：Y=25.789-0.656XT-41.958XAOD＋3.262XID（XT=10,20,30）。结论温度及反映DNA含量的各个参数与PMI存在较强的相关性,可以将温度作为参数引入方程用于推断PMI。 |
| 关 键 词： | 法医病理学 死后间隔时间 图像分析技术 人肝细胞 DNA降解 |
Early PMI estimation by DNA degradation of human hepatocytes at different temperatures |
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| LI Shanshan,ZHAO Chunming,LIU Quan,LIU Liang. Early PMI estimation by DNA degradation of human hepatocytes at different temperatures[J]. Chinese Journal of Forensic Medicine, 2011, 26(1): 4-6 | |
| Authors: | LI Shanshan ZHAO Chunming LIU Quan LIU Liang |
| Affiliation: | (LI Shanshan1,ZHAO Chunming1,LIU Quan2,LIU Liang3 1.Department of Pathology,Qiqihaer Medical College,Heilongjiang Qiqihaer 161006,China,2.Hubei University of Police,Wuhan 430030,3.Key Laboratory of Evidence Science,China University of Political Science and Law,Ministry of Education,Beijing 100088,China) |
| Abstract: | Objective To conduct a quantitative measurement of DNA degradation of human hepatocytes by image analysis technique and attempt to formulate temperature-related multiple regression equation.Methods The livers were selected from 13 cadavers whose definite PMI were known ranging from 13～34h.Samples were taken from the livers,and smeared every hour at10℃,20℃ and 30℃,respectively.The smears were stained by Feulgen-van's method.Geometric parameter(ID) and gray parameter(AOD) were chosen as the indices for analysis.Multi-variant regression analysis was performed for the parameters in relation to different temperatures.Results Regression equation related with temperature:Y=25.789-0.656XT-41.958XAOD+3.262XID(XT=10,20,30).Conclusion All the parameters examined for DNA degradation are closely related with the corresponding PMI.These parameters may be useful for estimation of PMI. |
| Keywords: | forensic pathology postmortem interval image analysis technology liver DNA degradation |
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