猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断

目的 研究猪眼玻璃体液成分变化及其与死亡时间(postmortem interval,PMI)的关系.方法放血处死家猪后取带眼睑的眼球96只,随机分为24组,在避光、温度(15±2)℃、湿度(50±5)%条件下分别放置2～96h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤(hy...

Concentration changes of potassium and hypoxanthine in vitreous humor of swine and its application to postmortem interval estimation

Objective To investigate the relationship between postmortem interval（PMI） and concentration changes of components in swine vitreous humor.Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage,being randomly divided into 24 groups,were stored in dark situation,at temperature of（15±2） ℃ and humidity of（50±5）% for 2-96 hours separately.The vitreous humor was collected.Concentrations of K＋,Na＋,Cl-and hypoxanthine（Hx） were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph（UPLC）.The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K＋ and Hx were positively correlated with PMI（R2=0.767 and R2=0.793,respectively）.Binary linear regression showed a higher correlation for K＋ and Hx with PMI estimation（R2=0.866）.PMI was not significantly correlated with vitreous Na＋ and Cl-concentrations.Conclusion Vitreous K＋ and Hx concentrations can be used as the objective markers for PMI estimation.The binary linear regression functions of vitreous K＋ and Hx concentrations with PMI are more accurate for estimating the PMI.