| 猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断 |
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| 引用本文： | Fang C,Wang SC,Sun LM,Zhang XT,Long WQ,Jing HL. 猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断[J]. 法医学杂志, 2011, 27(1): 9-12, 21. DOI: 10.3969/j.issn.1004-5619.2011.01.003 |
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| 作者姓名： | Fang C Wang SC Sun LM Zhang XT Long WQ Jing HL |
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| 作者单位： | 中山大学中山医学院法医病理教研室;广州市刑事科学技术研究所;中山大学附属第一医院检验科; |
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| 摘 要： | 目的 研究猪眼玻璃体液成分变化及其与死亡时间(postmortem interval,PMI)的关系.方法放血处死家猪后取带眼睑的眼球96只,随机分为24组,在避光、温度(15±2)℃、湿度(50±5)%条件下分别放置2～96h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤(hy...
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| 关 键 词： | 法医病理学 次黄嘌呤 钾 玻璃体液 死亡时间 猪 |
Concentration changes of potassium and hypoxanthine in vitreous humor of swine and its application to postmortem interval estimation |
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| Fang Chao,Wang Song-cai,Sun Li-min,Zhang Xiao-ting,Long Wei-qing,Jing Hua-lan. Concentration changes of potassium and hypoxanthine in vitreous humor of swine and its application to postmortem interval estimation[J]. Journal of Forensic Medicine, 2011, 27(1): 9-12, 21. DOI: 10.3969/j.issn.1004-5619.2011.01.003 |
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| Authors: | Fang Chao Wang Song-cai Sun Li-min Zhang Xiao-ting Long Wei-qing Jing Hua-lan |
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| Affiliation: | FANG Chao1,WANG Song-cai2,SUN Li-min2,ZHANG Xiao-ting2,LONG Wei-qing3,JING Hua-lan1(1.Department of Forensic Pathology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China,2.Guangzhou Criminal Science and Technology Institute,Guangzhou 510030,3.Department of Laboratory Medicine,The First Affiliated Hospital of Sun Yat-sen University,China) |
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| Abstract: | Objective To investigate the relationship between postmortem interval（PMI） and concentration changes of components in swine vitreous humor.Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage,being randomly divided into 24 groups,were stored in dark situation,at temperature of（15±2） ℃ and humidity of（50±5）% for 2-96 hours separately.The vitreous humor was collected.Concentrations of K＋,Na＋,Cl-and hypoxanthine（Hx） were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph（UPLC）.The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K＋ and Hx were positively correlated with PMI（R2=0.767 and R2=0.793,respectively）.Binary linear regression showed a higher correlation for K＋ and Hx with PMI estimation（R2=0.866）.PMI was not significantly correlated with vitreous Na＋ and Cl-concentrations.Conclusion Vitreous K＋ and Hx concentrations can be used as the objective markers for PMI estimation.The binary linear regression functions of vitreous K＋ and Hx concentrations with PMI are more accurate for estimating the PMI. |
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| Keywords: | forensic pathology hypoxanthine potassium vitreous humor postmortem interval swine |
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