灌服毒鼠强诱导大鼠细胞DNA的损伤研究

目的研究毒鼠强体内染毒后,毒鼠强对大鼠脑细胞、心肌细胞、淋巴细胞DNA的损伤作用。方法选择健康Sprague-Dawley大白鼠20只,分成5组,每组4只,采用灌胃方法使大鼠毒鼠强体内染毒,按0.2,0.1,0.05,0.01mg·kg-1制作大鼠毒鼠强中毒模型,并以灌服生理盐水的健康大鼠为对照,分离实验大鼠的淋巴细胞、心肌细胞和脑细胞,用彗星电泳的方法测定不同浓度毒鼠强中毒后的细胞DNA损伤。结果0.2,0.1,0.05,0.01mg·kg-1剂量组的毒鼠强均可引起大鼠的淋巴细胞、心肌细胞和脑细胞DNA损伤,均与对照组差异有显著性(P<0.01)。结论毒鼠强诱导体内细胞DNA损伤可能是毒鼠强毒性作用机制之一。

Detecting DNA damage of cell in rats using comet assay after tetramine poisoned

OBJECTIVE: To study the damage of DNA in lymphocytes, brain cells and cardiac muscle cells of rats induced by different dose of tetramine and to speculate the toxicant mechanism of tetramine. METHODS: The rat were poisoned by Tetramine, which was taken in by mouth. The rat poisoning models were used by 0.2, 0.1, 0.05, 0.01 mg x kg(-1) Tetramine, and comparison model was made by NS. Lymphocytes and brain cells and cardiac muscle cells of rats were separatd and collected form experimentation rat. DNA damages of cells which were exposed to different doses of tetramine were detected using the single cell gel electrophorresis (SCGE) or comet assay. RESULTS: DNA damages have been observed in lymphocytes, brain cells and cardiac muscle cells of rats which exposed form 0.01mg x kg(-1) doses of tetramine to 0.2mg x kg(-1) doses of tetramine. The test groups are very significantly statistical different to the control group (P<0.01). CONCLUSION: It is assumed that DNA damages of cells might be one of the toxicant mechanism of tetramine.