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Sex identification in fresh blood and dried bloodstains by a nonisotopic deoxyribonucleic acid (DNA) analyzing technique
Authors:R Kobayashi  H Nakauchi  Y Nakahori  Y Nakagome  S Matsuzawa
Institution:Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Abstract:Deoxyribonucleic acid (DNA) specimens were prepared from blood or bloodstain extracts, and the content of a Y-chromosome specific DNA fragment was investigated by the Southern hybridization method using a nonisotopic staining technique. Thus obtained patterns of male DNA showed a clear band, whereas broad stains with some faint bands appeared on the patterns of DNA from both sexes. This method is expected to be a new powerful mean of forensic medical examination.
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