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| 依据共有STR基因座数判别全同胞关系 | |
| 引用本文： | 方建新,赵书民,李成涛,张素华,李莉. 依据共有STR基因座数判别全同胞关系[J]. 法医学杂志, 2009, 25(5): 341-344. DOI: 10.3969/j.issn.1004-5619.2009.05.007 |
| 作者姓名： | 方建新 赵书民 李成涛 张素华 李莉 |
| 作者单位： | 1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海,200063 2. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海,200063;复旦大学生命科学学院,上海,200433 3. 苏州大学医学部法医学系,江苏,苏州,215123 |
| 基金项目： | 中央级科研院所社会公益研究资助项目 |
| 摘 要： | 目的建立并探讨基于共有STR基因座数的全同胞关系判别方法。方法根据280对全同胞（fullsibling,FS）及2 003对无关个体（unrelated individual,UI）Identifiler系统15个STR基因座的分型结果,采用计数法计算全不同基因座数（A0）、半相同基因座数（A1）和全相同基因座数（A2）,依据ITO法计算每对受试者的全同胞指数（FSI）,应用判别分析得出基于共有基因座数或FSI进行全同胞及无关个体关系判别的Fisher判别函数,并比较其判别效能。结果全同胞对中的A1、A2和无关个体对中的A0、A1均呈正态分布,全同胞对中的A0和无关个体对中的A2均呈偏态分布。A1在两组人群中的分布差异无统计学意义（P〉0.01）。同时采用A0和A2建立的全同胞及无关个体关系的判别函数分别为ZFS=0.99817A0＋4.24442A2-12.77970和ZUI=2.014 56 A0＋1.546 58 A2-7.280 76。采用上述判别函数进行全同胞及无关个体关系判别的平均错判率为0.049 0。上述判别函数的判别效能与基于FSI的判别函数的判别效能差异无统计学意义。结论可以采用Identifiler系统的共有基因座数进行全同胞及无关个体关系的判别,所建立的判别公式的判别效能与经典ITO法相近。 |
| 关 键 词： | 法医遗传学 同胞关系 短串联重复序列 判别分析 共有基因座 |
Full Sibling Identification Based on the Number of Matched STR Locus |
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| FANG Jian-xin,ZHAO Shu-min,LI Cheng-tao,ZHANG Su-hua,LI Li. Full Sibling Identification Based on the Number of Matched STR Locus[J]. Journal of Forensic Medicine, 2009, 25(5): 341-344. DOI: 10.3969/j.issn.1004-5619.2009.05.007 | |
| Authors: | FANG Jian-xin ZHAO Shu-min LI Cheng-tao ZHANG Su-hua LI Li |
| Affiliation: | FANG Jian-xin1,ZHAO Shu-min1,LI Cheng-tao1,2,ZHANG Su-hua3,LI Li1(1.Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China,2.School of Life Sciences,Fudan University,Shanghai 200433,3.De-partment of Forensic Medicine,Medical College of Soochow University,Suzhou 215123,China) |
| Abstract: | Objective To establish and evaluate the method of matched locus numbers in full sibling identifica-tion.Methods Two hundred and eighty full sibling(FS) pairs and 2 003 unrelated individual(UI) pairs were genotyped with Identifiler system.The number of locus matched with 0 identical allele(A0),matched with 1 allele(A1) or matched with 2 alleles(A2) were counted and full sibling index(FSI) were calculated based on ITO method.Fisher discriminant functions were established based on the numbers of matched STR lo... |
| Keywords: | forensic genetics sibling relations short tandem repeat discriminant analysis matched locus |
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