电击死大鼠心肌细胞磷脂变化的研究

目的探讨无电流斑与有电流斑电击死心肌组织磷脂分布。方法 15只SD大鼠,无电流斑和有电流斑各6只,对照组3只。实验组通过自制电击装置通电致死,对照组采用脱颈处死。实验组和对照组大鼠死后0h、12h、24h三个时间段取心肌组织固定和冷冻,对其分别进行制片HE染色和磷脂染色。结果电击死均可见心肌纤维断裂、波浪状改变,血管内皮细胞极性化改变,部分心肌间质少量出血。磷脂染色:电击死0h、12h心肌细胞膜磷脂缺失,随时间延长,磷脂缺失程度加重。对照组仅可偶见单个磷脂位于细胞间质或少量心肌细胞膜磷脂缺损。死后24h,电击组、对照组均可见大面积心肌细胞膜磷脂缺失。结论心肌细胞膜磷脂染色可有助于电击死诊断,但死亡时间达到24h左右则诊断价值不大。

Changes of phospholipids in cardiomyocytes after electric fatalities in rats

Objective To investigate the distribution of phospholipids in myocardial tissue induced by electric fatalities without and with electric mark. Methods A total of 15 SD rats were divided into 3 groups: 6 rats without electric mark, 6 rats with electric mark, and 3 rats in the control group, the experimental groups were electrified by self-made electric shock device, and the control group was killed by cervical dislocation. Myocardial tissue of rats in the experimental groups and the control group were fixed and frozen at 0 h, 12 h and 24 h after death, and were stained with HE staining and phospholipid staining. Results Myocardial fiber rupture, wavy changes, vascular endothelial cell polarity changes, a small amount of myocardial interstitial bleeding can be seen in all the electric fatalities. Phospholipid staining: Phospholipids were lost in myocardial cells at 0 h and 12 h after electric fatalities, and the degree of phospholipid deficiency was aggravated with time.In the control group, only a single phospholipid was found to be located in the interstitial cell or a small amount of phospholipid defects in the myocardial cell membrane. At 24 hours after death, large areas of myocardial cell membrane phospholipid deficiency were seen in both the electroshock group and the control group. Conclusion Myocardial cell membrane phospholipid staining may be helpful in the diagnosis of electric fatalities, but the diagnostic value is not significant when the time of death is over 24 hours.