| 质谱法分析水及三种生物基质中的敌草快 |
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| 引用本文： | 赵森,王炯,于忠山.质谱法分析水及三种生物基质中的敌草快[J].中国法医学杂志,2020(3):274-277. |
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| 作者姓名： | 赵森 王炯 于忠山 |
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| 作者单位： | 浙江警察学院;公安部物证鉴定中心 |
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| 基金项目： | 浙江省毒品防控技术研究重点实验室开放课题《基于城市污水流行病学法进行毒情分析和溯源研究》(2019005)。 |
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| 摘 要： | 目的建立了超高效液相色谱——质谱法(UPLC-MS/MS)测定环境样品水基质以及包括人血液、尿液、肝脏的生物样品中敌草快的检测方法。方法空白水基质、空白血、空白尿、空白肝加入敌草快标准溶液,经混合型弱阳离子交换柱(WCX)进行纯化,提取后采用UPLC-MS/MS检测。质谱检测采用正离子扫描,多反应离子监测模式(MRM)。结果空白水、空白血、空白尿、空白肝加标回收率在70%~100%,检出限均为0.1ng/mL。结论该方法灵敏度高,分析速度快,操作简便。
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| 关 键 词： | 敌草快 超高效液相色谱——质谱法 环境样品 生物基质 |
Determination of Diquat in blank water and three blank biological matrix with UPLC-MS/MS |
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| Zhao Sen,Wang Jiong,Yu Zhongshan.Determination of Diquat in blank water and three blank biological matrix with UPLC-MS/MS[J].Chinese Journal of Forensic Medicine,2020(3):274-277. |
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| Authors: | Zhao Sen Wang Jiong Yu Zhongshan |
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| Institution: | (Zhejiang Police College,Hangzhou,310053;Institute of Forensic Science of China,Beijing,100038) |
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| Abstract: | Objective To establish a UPLC-MS/MS method for the quantitative determination and confirmation of Diquat.Methods The blank water,blank whole blood,blank urine and blank liver were vortexed and centrifuged.The supernatant was cleaned-up using Oasis®WCX solid phase extraction cartridges.Extracts were analyzed by UPLC-MS/MS in positive ionization and multiple reaction monitoring(MRM)mode.Results The average recovery of Diquat from the blank water,blank whole blood,blank urine and blank liver were 70%~100%.The detection limit was 0.10ng/mL.Conclusion The method is proved to be selective,sensitive and rapid. |
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| Keywords: | Diquat UPLC-MS/MS Environmental sample Biological matrix |
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