| mRNA在体液斑鉴定研究中内参基因的选择与应用 |
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| 引用本文： | 赵一霞,季安全,胡胜,孙启凡. mRNA在体液斑鉴定研究中内参基因的选择与应用[J]. 中国法医学杂志, 2020, 0(2): 196-200,191 |
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| 作者姓名： | 赵一霞 季安全 胡胜 孙启凡 |
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| 作者单位： | 公安部物证鉴定中心现场物证溯源技术国家工程实验室法医遗传学公安部重点实验室 |
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| 基金项目： | 基本科研业务费(No.2018JB006,No.2018JB036);国家重点研发计划课题(No.2017YFC0803503)。 |
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| 摘 要： | 利用mRNA分析技术鉴定生物检材中各种体液斑迹的组织属性来源是近年来法医物证学的一个重要进展。终点逆转录PCR和实时荧光定量PCR技术是法医学实验室常用的mRNA检测技术,为保证实验结果的准确性,研究者通常选用组成性表达的管家基因作为内参,对不同样本提取RNA进行质控和标准化,以正确评估目标mRNA在各样本中的表达量。本文聚焦近年来使用mRNA分析技术进行体液斑迹组织属性来源鉴定的研究报道,对这些工作中内参基因的选择与应用效果进行综述。
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| 关 键 词： | 法医物证学 MRNA 内参基因 体液斑迹鉴定 |
Selection and application of internal reference genes in m RNA profiling for the identification of forensic body fluids |
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| Zhao Yixia,Ji Anquan,Hu Sheng,Sun Qifan. Selection and application of internal reference genes in m RNA profiling for the identification of forensic body fluids[J]. Chinese Journal of Forensic Medicine, 2020, 0(2): 196-200,191 |
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| Authors: | Zhao Yixia Ji Anquan Hu Sheng Sun Qifan |
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| Affiliation: | (MPS’s Key Laboratory of Forensic Genetics,National Engineering Laboratory for Crime Scene Evidence Investigation and Examination,Institute of Forensic Science,Ministry of Public Security(MPS),Beijing 100038,China) |
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| Abstract: | mRNA profiling has been under scrutiny over the past couple of years for its potential ability to identify a variety of body fluids in forensic samples. Endpoint PCR and Real-time fluorescence quantitative PCR are common mRNA semi-quantitative and quantitative detection techniques used in forensic laboratories. In these experiments, constitutively expressed housekeeping genes would be used as a reference for RNA quality control and normalization, for assessing the expression of target mRNA from various biological samples. This paper mainly reviews the selection and application of internal reference genes in the mRNA profiling for the identification of biological fluids. |
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| Keywords: | forensic biological evidence mRNA internal reference genes body fluids identification |
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