小鼠皮肤创伤愈合过程中IL-10在不同表达部位的组化定量研究

目的探讨小鼠皮肤创伤愈合过程中IL-10在不同表达部位的变化与损伤时间的关系。方法应用免疫组织化学技术和形态计量法,对小鼠不同时程皮肤切创组织中IL-10不同表达部位(表皮细胞及表皮下组织的浸润细胞)的变化进行研究。结果皮肤切创前后表皮层均有阳性表达,切创后1～3h表皮细胞阳性表达水平迅速升高,24h时降至较低水平,伤后48h表达水平再次升高,96h后逐渐回复到正常水平;表皮以下阳性部位主要是浸润的单核/巨噬细胞,损伤6h后阳性细胞在真皮、皮下组织及创口肉芽组织内逐渐增多,于伤后72h达最大值(51.41±3.12)%,96～168h阳性细胞数逐渐减少(29.38±2.64)%～(5.56±4.74)%。结论皮肤创伤修复过程中IL-10在不同表达部位的变化特点与皮肤损伤时间相关,检测其表达变化可望用于损伤时间推断。

Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice

OBJECTIVE: To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice. METHODS: Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. RESULTS: In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1-3h after incision, and it returned to lower lever by 24h, but increased again at 48h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6h after incision, and the maximum ratio was (51.41 +/- 3.12)% in a 72h-old wound. Then the positive cells gradually decreased (29.38 +/- 2.64)%-(5.56 +/- 4.74)% after incision 96-168h. CONCLUSIONS: The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.