鸡传染性腺胃炎病原的分离鉴定

河北省某个体户蛋用种鸡场连续孵化出的7批海赛商品雏鸡共6万余只,在30～80日龄时发生一种以极度消瘦、腺胃肿大为特征的疾病.采集发病鸡的腺胃进行病原分离鉴定,从中分离出传染性支气管炎病毒(IBV).将发病鸡的腺胃匀浆乳剂及第5代鸡胚分离物接种SPF鸡,结果用鸡腺胃匀浆乳剂接种的鸡出现了与自然病鸡相同的临床症状,而用第5代鸡胚分离物接种的鸡未能复制出腺胃肿大病例.取各接种组鸡血清进行17种相关病原抗体检测,结果接种病鸡腺胃匀浆乳剂的鸡检出了网状内皮组织增生病病毒(REV)抗体.初步试验结果表明,此次发生的以腺胃肿大为特征的疫病可能与REV感染有关,而所分离到的IBV本身并不能引起腺胃肿大.

Isolation and identification of the pathogens causing infectious proventriculitis in chicks

Seven batchssof six ten thousand egg used chicks were incubated on an egg used breeder chicks farm, Hebei province, where a disease characterized by serious leaning sign and swollen proventriculus happened in the chicks at age of 30 to 80 day. And an infectious bronchitis virus (IBV) was isolated from the proventriculus sampled from the affected chicks. SPF chicks were inoculated with the homogenate of the proventriculus from the affected chicks and the isolate of the fifth passages chick embryos, respectively. And the chicks inoculated the homogenate of the proventriculus showed clinical signs as same as the natural case but the chicks innoculated with the isolate of the fifth passages chick embryos didn't showed any lesions of swollen proventriculus. And then the serum specimens were sampled from the chicks of the tested groups for examining the antibodies related with 17 pathogens, the test result revealed that the antibody to reticuloendotheliosis virus (REV) existed in the sera from the chicks inoculated with the homogenate of the proventriculus. The preliminary test result indicated that this disease characterized by swollen proventriculus was possiblely related with infection by REV. However, the isolate of IBV wasn's able to cause any swollen proventriculus lesion of the affected chicks.