应用酶组织化学方法鉴定死亡时间的初步探讨

利用7例颅脑外伤死亡的健康青年尸体,在死后48h,环境温度18～24℃,空气相对湿度83～92％和实验湿度54～64％的条件下,检测肝脏、肾脏酶活性的变化。实验结果表明,肝脏乳酸脱氢酶(LDH)和L-苹果酸脱氢酶(MDH),随着死亡时间的延长,活性逐渐减低,48h近于阴性;而肾脏上述二种酶活性则在死亡后6h和24h出现高峰,36h开始下降;肝脏的酸性磷酸酶(ACP)亦于死后6h和24h出现高峰,36h开始下降。而肾脏此种酶在死后18～24h,有增高趋势。笔者认为上述酶活性的规律性变化有助于死亡时间的推断。应用二种以上酶活性的变化特点,能够较准确地判断死亡时间。

A STUDY OF THE IDENTIFICATION OF TIME ELAPSED AFTER DEATH WITH ENZYMOHISTOCHEMISTRY

A study of enzymohistochemical changes of tissues from seven healthy young corpses died of cranial injuries is reported. Within 48h after death, under the condition of enviromental temperature 18～24℃, relarive air humidity 83～92％ and experimental humidity 54～64％. changes of the enzyme activity occurred in livers and kidneys were recorded. The activities of hepatic lactate dehydrogenase (LDH) and malic dehydrogenase (MDH) reduced with the prologation of the time elapsed after death , Almost no enzyme activities were obscrued 48h after death. But in kidney,the activities of these two enzymes reached to their Peaks 6 or 24h after death and then reduced from 36h. The activity of hepatic acid phosphatase (ACP) increased from 18 to 24h. The authors believe that the changes in enzyme activity are valuable in identification of the time of death, and if the activeties of two or more enzymes are studied, the time of death could be identified accurately.